B7-H6 Protein (AA 25-262) (His tag,AVI tag,Biotin)

Produktnummer ABIN5674584

Produktdetails

Target: B7-H6 (NCR3LG1) (Natural Killer Cell Cytotoxicity Receptor 3 Ligand 1 (NCR3LG1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 25-262

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses B7-H6 Protein ist gelabelt mit His tag,AVI tag,Biotin.

Marke: PrecisionAvi

Sequenz: AA 25-262

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Produktmerkmale: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 30.4 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Reinheit: >90 % as determined by reduced SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Kommentare:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Antigendetails

Target: B7-H6 (NCR3LG1) (Natural Killer Cell Cytotoxicity Receptor 3 Ligand 1 (NCR3LG1))

Andere Bezeichnung: B7-H6 (NCR3LG1 Produkte)

Synonyme: B7-H6 Protein, B7H6 Protein, DKFZp686O24166 Protein, natural killer cell cytotoxicity receptor 3 ligand 1 Protein, NCR3LG1 Protein

Hintergrund: The B7 family of genes is essential in the regulation of the adaptive immune system. one of which is the recently discovered B7H6. Humans and rats have a single B7H6 gene, however, many B7H6 genes were detected in a single large cluster in the Xenopus genome. Chimeric antigen receptor (CAR) T-cell therapies have demonstrated durable and potentially curative therapeutic efficacy against B-cell leukemia in clinical trials. In this study, B7H6, a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.

Molekulargewicht: 30.4 kDa