MMP13 Protein (AA 14-466) (His tag)

Produktnummer ABIN5665876

Produktdetails

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Biologische Aktivität: Active

Proteineigenschaft: AA 14-466

Spezies: Ratte

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses MMP13 Protein ist gelabelt mit His tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 97 %

Biological Activity Comment: Matrix Metalloproteinase 13 (MMP13) is a member of the matrix metalloproteinase (MMP) family. MMP13 has been proposed to participate in aggrecan degradation associated with osteoarthritis and cleavage of type II collagen in osteoarthritic cartilage explants and in tumor progression and metastasis. In addition, it can cleave type I, III, IV, IX, X and XIV collagens and fibronectin. MMP13 is likely to play a crucial role in the modulation of extracellular matrix degradation and cell-matrix interactions. Although gelatin zymography is mainly used for the detection of the MMP2 and MMP9, it also can be used for MMP13 dection. Briefly, various concentrations of MMP13 (1000ng, 500ng, 250ng, 125ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphat- polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP13 would hydrolyze gelatin nearby, which was indicated by the white bands on the gel. In this experiment we use heat-denatured MMP13 protein as negative control, and blood sample as positive control. Result: Gelatin hydrolysis by recombinant rat MMP13 was shown in figure 1 Gelatin hydrolysis by recombinant rat MMP13.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 5.1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Andere Bezeichnung: Matrix Metalloproteinase 13 (MMP13) (MMP13 Produkte)

Synonyme: CLG3 Protein, MANDP1 Protein, Clg Protein, MMP-13 Protein, Mmp1 Protein, cb1034 Protein, mmp13 Protein, gene A Protein, xCol Protein, xcl3 Protein, MMP13 Protein, MGC108008 Protein, matrix metallopeptidase 13 Protein, matrix metallopeptidase 13a Protein, matrix metallopeptidase 13 (collagenase 3) like S homeolog Protein, matrix metallopeptidase 13 (collagenase 3) Protein, matrix metalloproteinase 13 Protein, matrix metallopeptidase 13 (collagenase 3) like L homeolog Protein, MMP13 Protein, Mmp13 Protein, mmp13a Protein, mmp13l.S Protein, mmp13 Protein, LOC100136348 Protein, mmp13l.L Protein

Hintergrund: Alternative Names: CLG3, Collagenase 3

Molekulargewicht: 60kDa

UniProt: P23097