UCHL3 Protein (partial) (His tag)

Produktnummer ABIN5608315

Produktdetails

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Protein-Typ: Recombinant

Proteineigenschaft: partial

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses UCHL3 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB)

Sequenz: Glu 2 - Ala 230

Reinheit: >95 % as determined by SDS-PAGE.

Endotoxin-Niveau: Endotoxin level is less than 1.0 EU per ug by the LAL method.

Anwendungsinformationen

Applikationshinweise: This recombinant protein can be used for WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 50 mM Tris, 150 mM NaCl, pH 7.5

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C.

Antigendetails

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Andere Bezeichnung: UCH-L3 (Uchl3 Produkte)

Synonyme: UCH-L3 Protein, UCH-6 Protein, RGD1561196 Protein, im:6908825 Protein, zgc:109963 Protein, uchl4 Protein, ubiquitin C-terminal hydrolase L3 Protein, ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) Protein, ubiquitin C-terminal hydrolase L3 L homeolog Protein, UCHL3 Protein, Uchl3 Protein, uchl3 Protein, uchl3.L Protein

Hintergrund: Ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCH-L3), a member of peptidase C12 family, is also known as ubiquitin thioesterase L3. Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. UCH-L3 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCH-L3 indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is also required for stress-response retinal, skeletal muscle and germ cell maintenance. Furthermore, UCH-L3 may be involved in working memory and can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.

Molekulargewicht: 27 kDa

Gen-ID: 7347

NCBI Accession: NP_005993

Pathways: Feeding Behaviour, Positive Regulation of fat Cell Differentiation