SERPINA1 Protein (AA 25-418) (His tag)

Produktnummer ABIN4370407

Produktdetails

Target: SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 25-418

Spezies: Human

Quelle: Human Cells

Aufreinigungstag / Konjugat: Dieses SERPINA1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Serpin A1/alpha-1-Antitrypsin (C-6His)

Sequenz: EDPQGDAAQK TDTSHHDQDH PTFNKITPNL AEFAFSLYRQ LAHQSNSTNI FFSPVSIATA FAMLSLGTKA DTHDEILEGL NFNLTEIPEA QIHEGFQELL RTLNQPDSQL QLTTGNGLFL SEGLKLVDKF LEDVKKLYHS EAFTVNFGDT EEAKKQINDY VEKGTQGKIV DLVKELDRDT VFALVNYIFF KGKWERPFEV KDTEEEDFHV DQVTTVKVPM MKRLGMFNIQ HCKKLSSWVL LMKYLGNATA IFFLPDEGKL QHLENELTHD IITKFLENED RRSASLHLPK LSITGTYDLK SVLGQLGITK VFSNGADLSG VTEEAPLKLS KAVHKAVLTI DEKGTEAAGA MFLEAIPMSI PPEVKFNKPF VFLMIDQNTK SPLFMGKVVN PTQKVDHHHH HH

Produktmerkmale: Recombinant Human Serpin A1 produced by transfected human cells is a secreted protein with sequence (Glu25-Lys418) of Human SERPIN A1 fused with a polyhistidine tag at the C-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 2 mM CaCl2, pH 7.5.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 3 months

Antigendetails

Target: SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))

Andere Bezeichnung: alpha-1-antitrypsin (SERPINA1 Produkte)

Synonyme: AACT Protein, ACT Protein, GIG25 Protein, A1AT Protein, PI Protein, alpha-1-PI Protein, A1A Protein, AAT Protein, PI1 Protein, PRO2275 Protein, alpha1AT Protein, Pi Protein, Spi1 Protein, MGC64429 Protein, serpina1d Protein, Aps Protein, SERPINA3-5 Protein, serpin family A member 3 Protein, alpha-1-antiproteinase F precursor Protein, serpin family A member 1 Protein, alpha-1-antitrypsin Protein, calumenin Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 S homeolog Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 Protein, SH2B adaptor protein 2 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 L homeolog Protein, SERPINA3 Protein, LOC100008973 Protein, SERPINA1 Protein, LOC100328621 Protein, CALU Protein, Serpina1 Protein, serpina1.S Protein, serpina1 Protein, SPIA5 Protein, Sh2b2 Protein, Calu Protein, serpina1.L Protein

Sub Type: Fusionprotein

Hintergrund: Serpin A1 is a prototype member of the Serpin superfamily of the serine protease inhibitors. As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in hepatocytes, and to a lesser extent, in macrophages as well as intestinal epithelial cell lines and secreted as the abundant proteinase inhibitor in the circulation whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Point mutations in the native SerpinA1 variants result in Serpin A1 deficiency, and consequently lead to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma. For example, the Z variants (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe SerpinA1 deficiency. Accordingly, Serpin A1 deficiency in circulation is associated with emphysema or liver disease.
Alternative Names: Alpha-1-Antitrypsin, Alpha-1 Protease Inhibitor, Alpha-1-Antiproteinase, Serpin A1, SERPINA1, AAT, PI

Molekulargewicht: 45.35 kDa

UniProt: P01009