ABCC6 Protein (AA 1-1498) (Strep Tag)

Produktnummer ABIN3137463

Produktdetails

Target: ABCC6 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 6 (ABCC6))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1498

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses ABCC6 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequenz: MNRGRSMATP GEQCAGLRVW NQTEQEPAAY HLLSLCFVRA ASSWVPPMYL WVLGPIYLLY IHRHGRCYLR MSHLFKTKMV LGLALILLYT FNVAVPLWRI HQGVPQAPEL LIHPTVWLTT MSFATFLIHM ERRKGVRSSG VLFGYWLLCC ILPGINTVQQ ASAGNFRQEP LHHLATYLCL SLVVAELVLS CLVDQPPFFS EDSQPLNPCP EAEASFPSKA MFWWASGLLW RGYKKLLGPK DLWSLGRENS SEELVSQLER EWRRSCNGLP GHKGHSSVGA PETEAFLQPE RSQRGPLLRA IWRVFRSTFL LGTLSLVISD AFRFAVPKLL SLFLEFMGDR NSSAWTGWLL AVLMFAAACL QTLFEQQHMY RAKVLQMRLR TAITGLVYRK VLVLSSGSRK SSAAGDVVNL VSVDIQRLAE SIIYLNGLWL LFLWIFVCFV YLWQLLGPSA LTAVAVFLSL LPLNFFITKK RGFHQEEQMR QKASRARLTS SMLRTVRTIK SHGWEHAFLE RLLHIRGQEL SALKTSTLLF SVSLVSFQVS TFLVALVVFA VHTLVAEDNA MDAEKAFVTL TVLSILNKAQ AFLPFSVHCI VQARVSFDRL AAFLCLEEVD PNGMIASNSR RSSKDRISVH NGTFAWSQES PPCLHGINLT VPQGCLLAVV GPVGAGKSSL LSALLGELLK VEGSVSIEGS VAYVPQEAWV QNTSVVENVC FRQELDLPWL QKVLDACALG SDVASFPAGV HTPIGEQGMN LSGGQKQRLS LARAVYKKAA IYLLDDPLAA LDAHVSQQVF KQVIGPSGLL QGTTRILVTH TLHVLPQADR ILVLANGTIA EMGSYQDLLQ RNGALVGLLD GARQPAGTHD AATSDDLGGF PGGGRPTCRP DRPRPTEAAP VKGRSTSEVQ MEASLDDPEA TGLTAEEDSV RYGRVKTTIY LSYLRAVGTP LCTYTLFLFL CQQVASFSQG YWLSLWADDP VVDGRQMHAA LRGWVFGLLG CLQAIGLFAS MAAVFLGGAR ASGLLFRSLL WDVARSPIGF FERTPVGNLL NRFSKETDTV DVDIPDKLRS LLTYAFGLLE VGLAVTMATP LAIVAILPLM VLYAGFQSLY VATSCQLRRL ESARYSSVCS HMAETFQGSL VVRAFRAQAS FTAQHDALMD ENQRVSFPKL VADRWLATNL ELLGNGLVFV AATCAVLSKA HLSAGLVGFS VSAALQVTQT LQWVVRSWTD LENSMVAVER VQDYARIPKE APWRLPTCAA QPLWPCGGQI EFRDFGLRHR PELPLAVQGV SLKIHAGEKV GIVGRTGAGK SSLAWGLLRL QEAAEGNIWI DGVPITHVGL HTLRSRITII PQDPVLFPGS LRMNLDLLQE HTDEGIWAAL ETVQLKAFVT SLPGQLQYEC AGQGDDLSVG QKQLLCLARA LLRKTQILIL DEATASVDPG TEMQMQAALE RWFTQCTVLL IAHRLRSVMD CARVLVMDEG QVAESGSPAQ LLAQKGLFYR LAHESGLA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ABCC6 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 6 (ABCC6))

Andere Bezeichnung: Abcc6 (ABCC6 Produkte)

Synonyme: ABC34 Protein, ARA Protein, EST349056 Protein, GACI2 Protein, MLP1 Protein, MOAT-E Protein, MOATE Protein, MRP6 Protein, PXE Protein, PXE1 Protein, URG7 Protein, Abcc1b Protein, DCC Protein, Dyscalc1 Protein, Mrp6 Protein, dyscalc Protein, ATP binding cassette subfamily C member 6 Protein, ATP-binding cassette, sub-family C (CFTR/MRP), member 6 Protein, ABCC6 Protein, Abcc6 Protein

Hintergrund: ATP-binding cassette sub-family C member 6 (EC 7.6.2.-) (EC 7.6.2.3) (Multidrug resistance-associated protein 6),FUNCTION: ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds, and xenobiotics from cells. Mediates ATP-dependent transport of glutathione conjugates such as leukotriene-c4 (LTC4) and N-ethylmaleimide S-glutathione (NEM-GS) (in vitro), and an anionic cyclopentapeptide endothelin antagonist, BQ-123. May contribute to regulate the transport of organic compounds in testes across the blood-testis-barrier (By similarity). {ECO:0000250|UniProtKB:O95255}., FUNCTION: Mediates the release of nucleoside triphosphates, predominantly ATP, into the circulation, where it is rapidly converted into AMP and the mineralization inhibitor inorganic pyrophosphate (PPi) by the ecto-enzyme ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1), therefore playing a role in PPi homeostasis. {ECO:0000250|UniProtKB:O95255}.

Molekulargewicht: 164.9 kDa

UniProt: Q9R1S7