PEAR1 Protein (AA 19-1034) (rho-1D4 tag)

Produktnummer ABIN3136767

Produktdetails

Target: PEAR1 (Platelet Endothelial Aggregation Receptor 1 (PEAR1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 19-1034

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses PEAR1 Protein ist gelabelt mit rho-1D4 tag.

Applikation: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: LNSNDPNVCT FWESFTTTTK ESHLRPFSLL PAESCHRPWE DPHTCAQPTV VYRTVYRQVV KMDSRPRLQC CRGYYESRGA CVPLCAQECV HGRCVAPNQC QCAPGWRGGD CSSECAPGMW GPQCDKFCHC GNNSSCDPKS GTCFCPSGLQ PPNCLQPCPA GHYGPACQFD CQCYGASCDP QDGACFCPPG RAGPSCNVPC SQGTDGFFCP RTYPCQNGGV PQGSQGSCSC PPGWMGVICS LPCPEGFHGP NCTQECRCHN GGLCDRFTGQ CHCAPGYIGD RCQEECPVGR FGQDCAETCD CAPGARCFPA NGACLCEHGF TGDRCTERLC PDGRYGLSCQ EPCTCDPEHS LSCHPMHGEC SCQPGWAGLH CNESCPQDTH GPGCQEHCLC LHGGLCLADS GLCRCAPGYT GPHCANLCPP DTYGINCSSR CSCENAIACS PIDGTCICKE GWQRGNCSVP CPLGTWGFNC NASCQCAHDG VCSPQTGACT CTPGWHGAHC QLPCPKGQFG EGCASVCDCD HSDGCDPVHG QCRCQAGWMG TRCHLPCPEG FWGANCSNTC TCKNGGTCVS ENGNCVCAPG FRGPSCQRPC PPGRYGKRCV QCKCNNNHSS CHPSDGTCSC LAGWTGPDCS EACPPGHWGL KCSQLCQCHH GGTCHPQDGS CICTPGWTGP NCLEGCPPRM FGVNCSQLCQ CDLGEMCHPE TGACVCPPGH SGADCKMGSQ ESFTIMPTSP VTHNSLGAVI GIAVLGTLVV ALIALFIGYR QWQKGKEHEH LAVAYSTGRL DGSDYVMPDV SPSYSHYYSN PSYHTLSQCS PNPPPPNKVP GSQLFVSSQA PERPSRAHGR ENHVTLPADW KHRREPHERG ASHLDRSYSC SYSHRNGPGP FCHKGPISEE GLGASVMSLS SENPYATIRD LPSLPGEPRE SGYVEMKGPP SVSPPRQSLH LRDRQQRQLQ PQRDSGTYEQ PSPLSHNEES LGSTPPLPPG LPPGHYDSPK NSHIPGHYDL PPVRHPPSPP SRRQDR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Pear1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PEAR1 (Platelet Endothelial Aggregation Receptor 1 (PEAR1))

Andere Bezeichnung: Pear1 (PEAR1 Produkte)

Synonyme: MEGF12 Protein, JEDI Protein, 3110045G13Rik Protein, Jedi-1 Protein, Megf12 Protein, RGD1305653 Protein, platelet endothelial aggregation receptor 1 Protein, PEAR1 Protein, pear1 Protein, Pear1 Protein

Hintergrund: When overexpressed, reduces the number of both early and late non-adherent myeloid progenitor cells. {ECO:0000269|PubMed:17226770}.

Molekulargewicht: 109.9 kDa Including tag.

UniProt: Q8VIK5