B3GNT1 Protein (AA 1-415) (Strep Tag)

Produktnummer ABIN3125379

Produktdetails

Target: B3GNT1 (UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 1 (B3GNT1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-415

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses B3GNT1 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MQMSYAIRCA FYQLLLAALM LVAMLQLLYL SLLSGLHGQE EQEQYFEFFP PSPRSVDQVK SQLRTALASG GVLDASGDYR VYRGLLKTTM DPNDVILATH ASVDNLLHLS GLLERWEGPL SVSVFAATKE EAQLATVLAY ALSSHCPEMR ARVAMHLVCP SRYEAAVPDP REPGEFALLR SCQEVFDKLA RVAQPGINYA LGTNTSYPNN LLRNLAREEA NYALVIDVDM VPSEGLWRGL REMLDQSNHW DGTALVVPAF EIRRSRRMPM NKNELVQLYQ VGEVRPFYYG LCTPCHAPTN YSRWVNLPEE SLLRPAYVVP WRDPWEPFYV AGGKVPTFDE RFRQYGFNRI SQACELHVAG FNFEVLNEGF LVHKGFKEAL KFHPQKEAEN QRNKILYRQF KQELKARYPN SPHRC
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: B3GNT1 (UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 1 (B3GNT1))

Andere Bezeichnung: B4gat1 (B3GNT1 Produkte)

Synonyme: B3GN-T2 Protein, B3GNT Protein, B3GNT-2 Protein, B3GNT1 Protein, BETA3GNT Protein, BGNT2 Protein, BGnT-2 Protein, B3GNT2 Protein, B3GN-T1 Protein, B3GNT6 Protein, BETA3GNTI Protein, MDDGA13 Protein, iGAT Protein, iGNT Protein, 1500032M01Rik Protein, B3gnt6 Protein, BETA3GNT1 Protein, B3gnt1 Protein, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Protein, beta-1,4-glucuronyltransferase 1 Protein, B3GNT2 Protein, B4GAT1 Protein, B4gat1 Protein

Hintergrund: Beta-1,4-glucuronyltransferase 1 (EC 2.4.1.-) (I-beta-1,3-N-acetylglucosaminyltransferase) (iGnT) (N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase) (Poly-N-acetyllactosamine extension enzyme) (UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1),FUNCTION: Beta-1,4-glucuronyltransferase involved in O-mannosylation of alpha-dystroglycan (DAG1) (PubMed:23217742, PubMed:25279699). Transfers a glucuronic acid (GlcA) residue onto a xylose (Xyl) acceptor to produce the glucuronyl-beta-1,4-xylose-beta disaccharide primer, which is further elongated by LARGE1, during synthesis of phosphorylated O-mannosyl glycan (PubMed:25279699). Phosphorylated O-mannosyl glycan is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed:25279699). Required for axon guidance, via its function in O-mannosylation of alpha-dystroglycan (DAG1) (PubMed:23217742). {ECO:0000269|PubMed:23217742, ECO:0000269|PubMed:25279699}.

Molekulargewicht: 47.4 kDa

UniProt: Q8BWP8

Pathways: Glycosaminoglycan Metabolic Process