SLC20A2 Protein (AA 1-656) (Strep Tag)

Produktnummer ABIN3136030

Produktdetails

Target: SLC20A2 (Solute Carrier Family 20 (Phosphate Transporter), Member 2 (SLC20A2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-656

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SLC20A2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MAMDGYLWMV ILGFIIAFIL AFSVGANDVA NSFGTAVGSG VVTLRQACIL ASIFETTGSV LLGAKVGETI RKGIIDVNLY NETVETLMAG EVSAMVGSAV WQLIASFLRL PISGTHCIVG STIGFSLVAI GPKGVQWMEL VKIVASWFIS PLLSGFMSGV LFILIRMFIL TKEDPVPNGL QALPLFYAAT IAINVFSIMY TGAPVLGLSL PIWAIALISF GVALLFAFFV WLFVCPWMKR KIAGRLEKES ALSRASDESL RKVQEAESPG FKELPGAKPS DDSAVPLTSL AGEAVGASEG TSAGNHPRAS YGRALSMTHG SAKSPISNGT FGFEGHMRND GHVYHTVHKD SGLYKDLLHK IHVDRGSEEK PTQENNYRLL RRNNSYTCYT AAICGMPVHT TFRASDTSSA PEDSEKLVGD SVSYSKKRLR YDSYSSYCNA VAEAEIEAEE GGVEMRLASE LADPDRPHED PTEEEKEEKD SAEVHLLFHF LQVLTACFGS FAHGGNDVSN AIGPLVALWL IYQQGGVTQE AATPVWLLFY GGVGICTGLW VWGRRVIQTM GKDLTPITPS SGFTIELASA FTVVIASNIG LPVSTTHCKV GSVVAVGWIR SRKAVDWRLF RNIFVAWFVT VPVAGLFSAA IMALLMYICG LFSSSR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SLC20A2 (Solute Carrier Family 20 (Phosphate Transporter), Member 2 (SLC20A2))

Andere Bezeichnung: Slc20a2 (SLC20A2 Produkte)

Synonyme: wu:fi23g11 Protein, zgc:152990 Protein, glvr-2 Protein, glvr2 Protein, mlvar Protein, pit-2 Protein, pit2 Protein, SLC20A2 Protein, GLVR-2 Protein, GLVR2 Protein, IBGC3 Protein, MLVAR Protein, PIT-2 Protein, PIT2 Protein, RAM1 Protein, Ab1-188 Protein, Glvr2 Protein, Pit-2 Protein, RAM-1 Protein, Ram1 Protein, MolPit2 Protein, Pit2 Protein, Ram-1 Protein, ChoPit2 Protein, HaPit2 Protein, PiT-2 Protein, solute carrier family 20 member 2 Protein, solute carrier family 20 (phosphate transporter), member 2 Protein, solute carrier family 20 (phosphate transporter), member 2 L homeolog Protein, solute carrier family 20, member 2 Protein, SLC20A2 Protein, slc20a2 Protein, slc20a2.L Protein, Slc20a2 Protein

Hintergrund: Sodium-dependent phosphate transporter 2 (Phosphate transporter 2) (PiT-2) (Solute carrier family 20 member 2) (Type III sodium-dependent phosphate transporter),FUNCTION: Sodium-phosphate symporter which preferentially transports the monovalent form of phosphate with a stoichiometry of two sodium ions per phosphate ion (PubMed:11003594, PubMed:23968976, PubMed:26822507, PubMed:30721528). Plays a critical role in the determination of bone quality and strength by providing phosphate for bone mineralization (PubMed:30721528). Required to maintain normal cerebrospinal fluid phosphate levels (PubMed:26822507). Mediates phosphate-induced calcification of vascular smooth muscle cells (VCMCs) and can functionally compensate for loss of SLC20A1 in VCMCs (PubMed:23968976). {ECO:0000269|PubMed:11003594, ECO:0000269|PubMed:23968976, ECO:0000269|PubMed:26822507, ECO:0000269|PubMed:30721528}.

Molekulargewicht: 70.9 kDa

UniProt: Q80UP8