GCS1 Protein (AA 1-834) (Strep Tag)

Produktnummer ABIN3124282

Produktdetails

Target: GCS1 (MOGS) (Mannosyl-Oligosaccharide Glucosidase (MOGS))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-834

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses GCS1 Protein ist gelabelt mit Strep Tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequenz: MARGERRRRA AAAEGARPLE RARAAGRRDG RAGGARGSAS GAALAVVVLA LAFGLSGRWV LAWLRVRRAL TLHPAPSALP PDSSSPAVAP ELFWGTYRPH VYFGMKTRSP KPLLTGLMWA QQGATPGTPP KLRHTCEQGD GVGPYGWEFH DGRTFGRQHI HDGALRLTTE FVKRPGGQHG GDWSWRVTVE PQASGTPSFP LVSLFFYVVT DGQEVLLPEI GAKGQLKSIS GHTSELGDFR LTLLPPTSPG DTVPKHGSYN VFWSSNPGLP QLTDMVKSRL NSWFQHRPPG ASPDRYLGLP GSLKWEERGP SGQGQFLIQQ VTLKAPFSVE FVFESGSAAT GGNQASGRLV GSQLTQALES HAAAFKERFE KTFQLKEKGL SPEEQALGQV ALSGLLGGIG YFYGQGLVLP DTSMEGSEQK MDPALFPPVP LFSGVPSRSF FPRGFLWDEG FHQLVVQRWD PHLTREALGH WLGLLNADGW IGREQILGDE ARARVPPEFL VQRAAHANPP TLLLPVVHML EGHDPDDLAF LRKAFPRLHA WFSWLHQSQA GPVPLSYRWR GRDLALPTLL NPKTLPSGLD DYPRASHPST AERHLDLRCW VALGARVLSQ LAEQLGETEA AAELGPLAAS LEEPGSLDEL HWAPELGVFA DFGNHTKAVQ LKSRPPQGLV RVVGRPPPRL QYVDALGYVS LFPLLLQLLD PSSPRLGPLL DVLADSRHLW SPFGLRSLSA SSLFYKQRNT EHDPPYWRGA VWLNINYLAL GALHHYGHVE GPHKVQAAKL YHELRANVVR NVRQQYQATG FLWEQYSDQD GRGMGCRPFQ GWTSLVLLIM AEEY
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: GCS1 (MOGS) (Mannosyl-Oligosaccharide Glucosidase (MOGS))

Andere Bezeichnung: Mogs (MOGS Produkte)

Synonyme: Afu6g04210 Protein, AO090701000141 Protein, Mogs Protein, CDG2B Protein, CWH41 Protein, DER7 Protein, GCS1 Protein, 1810017N02Rik Protein, AI181835 Protein, Gcs1 Protein, gcs1 Protein, im:7160827 Protein, wu:fe50a12 Protein, wu:fk09a10 Protein, zgc:158312 Protein, mannosyl-oligosaccharide glucosidase Protein, mannosyl-oligosaccharide glucosidase GCS1 Protein, mannosyl-oligosaccharide glucosidase L homeolog Protein, mannosyl oligosaccharide glucosidase Protein, glucosidase 1 Protein, AFUA_6G04210 Protein, Tc00.1047053511015.10 Protein, Tc00.1047053511805.10 Protein, LOC5576381 Protein, AOR_1_260114 Protein, MGYG_00305 Protein, TERG_01248 Protein, mogs.L Protein, TTHERM_00636930 Protein, LOAG_03690 Protein, Gcs1 Protein, MOGS Protein, Mogs Protein, mogs Protein

Hintergrund: Mannosyl-oligosaccharide glucosidase (EC 3.2.1.106) (Endoplasmic reticulum alpha-glucosidase I) (ER glu I) (Glucosidase 1) (Glycoprotein-processing glucosidase I),FUNCTION: In the context of N-glycan degradation, cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor in a highly specific manner. {ECO:0000250|UniProtKB:Q13724}., FUNCTION: (Microbial infection) Required for successful influenza or dengue virus infection, inhibition of its activity by a deoxynojirimycin derivative prevents death in mice infected with lethal doses of influenza or dengue viruses, even when administrated after infection. {ECO:0000269|PubMed:32227946}.

Molekulargewicht: 91.8 kDa

UniProt: Q80UM7

Pathways: SARS-CoV-2 Protein Interaktom