TRPM1 Protein (AA 1-1622) (Strep Tag)

Produktnummer ABIN3134946

Produktdetails

Target: TRPM1 (Transient Receptor Potential Cation Channel, Subfamily M, Member 1 (TRPM1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1622

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses TRPM1 Protein ist gelabelt mit Strep Tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequenz: MGSMRKMSSS FKRGSIKSST SGSQKGQKAW IEKTFCKREC IFVIPSTKDP NRCCCGQLTN QHIPPLPSGA PSTTGEDTKQ ADTQSGKWSV SKHTQSYPTD SYGILEFQGG GYSNKAMYIR VSYDTKPDSL LHLMVKDWQL ELPKLLISVH GGLQSFEMQP KLKQVFGKGL IKAAMTTGAW IFTGGVSTGV VSHVGDALKD HSSKSRGRLC AIGIAPWGMV ENKEDLIGKD VTRVYQTMSN PLSKLSVLNN SHTHFILADN GTLGKYGAEV KLRRQLEKHI SLQKINTRLG QGVPVVGLVV EGGPNVVSIV LEYLKEDPPV PVVVCDGSGR ASDILSFAHK YCDEGGVINE SLRDQLLVTI QKTFNYSKSQ SYQLFAIIME CMKKKELVTV FRMGSEGQQD VEMAILTALL KGTNASAPDQ LSLALAWNRV DIARSQIFVF GPHWPPLGSL APPVDTKATE KEKKPPTATT KGRGKGKGKK KGKVKEEVEE ETDPRKLELL NWVNALEQAM LDALVLDRVD FVKLLIENGV NMQHFLTIPR LEELYNTRLG PPNTLHLLVR DVKKSNLPPD YHISLIDIGL VLEYLMGGAY RCNYTRKSFR TLYNNLFGPK RPKALKLLGM EDDEPPAKGK KKKKKKKEEE IDIDVDDPAV SRFQYPFHEL MVWAVLMKRQ KMAVFLWQRG EECMAKALVA CKLYKAMAHE SSESELVDDI SQDLDNNSKD FGQLAVELLD QSYKHDEQVA MKLLTYELKN WSNSTCLKLA VAAKHRDFIA HTCSQMLLTD MWMGRLRMRK NPGLKVIMGI LIPPTILFLE FRTYDDFSYQ TSKENEDGKE KEEENVDANA DAGSRKGDEE NEHKKQRSIP IGTKICEFYN APIVKFWFYT ISYLGYLLLF NYVILVRMDG WPSPQEWIVI SYIVSLALEK IREILMSEPG KLSQKIKVWL QEYWNITDLV AISMFMVGAI LRLQSQPYMG YGRVIYCVDI ILWYIRVLDI FGVNKYLGPY VMMIGKMMID MLYFVVIMLV VLMSFGVARQ AILHPEEKPS WKLARNIFYM PYWMIYGEVF ADQIDLYAME INPPCGENLY DEEGKRLPPC IPGAWLTPAL MACYLLVANI LLVNLLIAVF NNTFFEVKSI SNQVWKFQRY QLIMTFHDRP VLPPPMIILS HIYIIIMRLS GRCRKKREGD QEERDRGLKL FLSDEELKKL HEFEEQCVQE HFREKEDEQQ SSSDERIRVT SERVENMSMR LEEINERENF MKTSLQTVDL RLSQLEELSG RMVSALENLA GIDRSDLIQA RSRASSECEA TYLLRQSSIN SADGYSLYRY HFNGEELLFE EPALSTSPGT AFRKKTYSFR VKDEDAKSHL DQPSNLHHTP GPSPPATPGR SRLALEGPLS TELRPGSDPG ISAGEFDPRA DFKSTEAAPS LNAAGVTGTQ LTVESTDSHP LRESKLVRYY PGDPNTYKTM KSRSFVYTEG RKLVRGLSNW SAEYSSIMDQ AWNATEWRCQ VQRITRSRST DIPYIVSEAA SQDELEDEHR GSLLDPQISR SALTVSDRPE KENLLSVKPH QTLGFPCLRS RSLHGRPRSA EPAPSKLDRA GHASSTSNLA VMSVVPEGQN TQQEKRSAET EC
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TRPM1 (Transient Receptor Potential Cation Channel, Subfamily M, Member 1 (TRPM1))

Andere Bezeichnung: Trpm1 (TRPM1 Produkte)

Synonyme: TRPM1b Protein, trpm1 Protein, zgc:158632 Protein, CSNB1C Protein, LTRPC1 Protein, MLSN1 Protein, 4732499L03Rik Protein, AI606771 Protein, Ltrpc1 Protein, Mlsn1 Protein, melastatin Protein, transient receptor potential cation channel subfamily M member 1 Protein, transient receptor potential cation channel, subfamily M, member 1a Protein, transient receptor potential cation channel, subfamily M, member 1 Protein, TRPM1 Protein, trpm1a Protein, LOC100541416 Protein, trpm1 Protein, Trpm1 Protein

Hintergrund: Transient receptor potential cation channel subfamily M member 1 (Long transient receptor potential channel 1) (LTrpC1) (Melastatin-1),FUNCTION: Forms nonselective divalent cation-conducting channels which mediate the influx of Na(+), Ca(2+), Mg(2+), Mn(2+), Ba(2+), and Ni(2+) into the cytoplasm, leading to membrane depolarization (By similarity). Impermeable to zinc ions (By similarity). In addition, forms heteromultimeric ion channels with TRPM3 which are permeable for calcium and zinc ions (By similarity). Essential for the depolarizing photoresponse of retinal ON bipolar cells. It is part of the GRM6 signaling cascade. Calcium channel which may play a role in metastasis suppression. May act as a spontaneously active, calcium-permeable plasma membrane channel. {ECO:0000250|UniProtKB:Q7Z4N2, ECO:0000269|PubMed:19861548, ECO:0000269|PubMed:19966281, ECO:0000269|PubMed:9537257}.

Molekulargewicht: 183.5 kDa

UniProt: Q2TV84