Contactin Associated Protein-Like 5C (CNTNAP5C) (AA 25-1305) protein (rho-1D4 tag)

Produktnummer ABIN3134887

Produktdetails

Target: Contactin Associated Protein-Like 5C (CNTNAP5C)

Protein-Typ: Recombinant

Proteineigenschaft: AA 25-1305

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: rho-1D4 tag

Applikation: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: ATNYNCDDPL TSFLSLRAFS SSSDLTGRSS PAQLNWRMGT GGWSPADSNA QQWLQMDLGN RVEITAVATQ GRYGSSDWVT SYRLMFSDTG HNWQQYTQEG SIWRFVGNMN ANSVVHHKLL NSVRARFVRF VPLEWNPNGK IGMRVEVYGC SYRSDVADFD GWSSLLYRFN QKTMSTLKDV ISLKFKSIQR DGVLFHGEGQ RGDHITLELQ NGRLALYLNL DDSKAQVSST APLATLGSLL DDQHWHSVLL ERVGKQANFT VDKNTQHFQT KGETDALDID YELSFGGIPV PSKPGTFLKK NFHGCIENLY YNGVNIIDLA KRRKHQIYSG NVTFSCSEPQ TVPITFVNSR SSYLLLTGTP QIDGLSVSFQ FRTWNEDGLL LSTELSEGSG TLLLILEGGT LRLLIKKLAR HGTEIFTGSG LNDGMWHSVS ISARRNRVTL TLDNDAASLP PDTSWLQIYS GNSYYFGGCP DNLTDSQCLN PIKAFQGCMR LIFIDNQPKD LISVQQGSLG SFSDLHIDLC SIKDRCLPNY CEHGGQCAQT WTNFYCNCSD TGYTGATCHD SIYEQSCEVY RHRGKTAEFF YVDSDGSGPL GPLQVFCNIT EDKIWMTVQH NNTGLTWVQG SNPEKPYAMT LNYGGSLEQL EALIDGSEHC EQEVTYYCKR SRLLNTPDGV PFTWWIGRSN EKHPYWGGSL PGVQQCGCGL EESCLDIRHF CNCDADTDEW TNDTGYLSFK DHLPVTQIII TDTNRSKSEA AWRIGPLRCY GDRHFWNAVS FSTEASFLHF PTFRVEFSAD IFFFFKTTAL SGVFLEILGI KDFLRLEMSS PSEVIFAIDV GNGPIDLLVQ SPYPLNDNQW HYIRAERNLK ETSLQVDNLP QSMREASEEG HFQFQLNSQL FVGGKSSRQK GFFGCIRSLH LNGQNIDLEE RAKVTSGVRP GCPGHCSSYG RNCQNGGKCV EKHIGYSCDC TNSPYEGPFC QKEISALFDS DTSVTYMFQE PYSVTKNTNL SSSAIYTDTA PSKEIIMLSF MTAQAPTLLL YLNFSSQNFL AILLSWNGSL QIHYQLSKEE SHVFTINTEN LANRRVHQVK MSRDGPELSI QMDQQLFSYT FSLESEFQRA RSLVLGKVTE TLGLDPEVAR ANTLGFVGCL SSVQYNHITP LKAALRHASI SPVTVQRTLT ESSCVSMVDS DANAVTTVYS STDPFGERDE REPLTNAVPS DLAVIGGIIA VVTFISFSVI GIMTHFFYQH KRSHYASQMK EKEYPENVDS SSRNDIDLQN TTRECKQEDF I
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Cntnap5c Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Contactin Associated Protein-Like 5C (CNTNAP5C)

Andere Bezeichnung: Cntnap5c (CNTNAP5C Produkte)

Synonyme: Caspr5-3 Protein, EG620292 Protein, Gm325 Protein, Caspr5-4 Protein, Cntnap5 Protein, Cntnap5d Protein, RGD1566343 Protein, contactin associated protein-like 5C Protein, Cntnap5c Protein

Hintergrund: May play a role in the correct development and proper functioning of the peripheral and central nervous system and be involved in cell adhesion and intercellular communication.

Molekulargewicht: 144.7 kDa Including tag.

UniProt: Q0V8T7