NEO1 Protein (AA 37-1493) (rho-1D4 tag)

Produktnummer ABIN3134731

Produktdetails

Target: NEO1 (Neogenin 1 (NEO1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 37-1493

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses NEO1 Protein ist gelabelt mit rho-1D4 tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: RPASGAAATK SGPRRQSQGA SVRTFTPFYF LVEPVDTLSV RGSSVILNCS AYSEPSPNIE WKKDGTFLNL ESDDRRQLLP DGSLFISNVV HSKHNKPDEG FYQCVATVDN LGTIVSRTAK LTVAGLPRFT SQPEPSSVYV GNSAILNCEV NADLVPFVRW EQNRQPLLLD DRIVKLPSGT LVISNATEGD GGLYRCIVES GGPPKFSDEA ELKVLQDPEE IVDLVFLMRP SSMMKVTGQS AVLPCVVSGL PAPVVRWMKN EEVLDTESSG RLVLLAGGCL EISDVTEDDA GTYFCIADNG NKTVEAQAEL TVQVPPGFLK QPANIYAHES MDIVFECEVT GKPTPTVKWV KNGDVVIPSD NFKIVKEHNL QVLGLVKSDE GFYQCIAEND VGNAQAGAQL IILEHDVAIP TLPPTSLTSA TTDHLAPATT GPLPSAPRDV VASLVSTRFI KLTWRTPASD PHGDNLTYSV FYTKEGVDRE RVENTSQPGE MQVTIQNLMP ATVYIFKVMA QNKHGSGESS APLRVETQPE VQLPGPAPNI RAYATSPTSI TVTWETPLSG NGEIQNYKLY YMEKGTDKEQ DIDVSSHSYT INGLKKYTEY SFRVVAYNKH GPGVSTQDVA VRTLSDVPSA APQNLSLEVR NSKSIVIHWQ PPSSTTQNGQ ITGYKIRYRK ASRKSDVTET LVTGTQLSQL IEGLDRGTEY NFRVAALTVN GTGPATDWLS AETFESDLDE TRVPEVPSSL HVRPLVTSIV VSWTPPENQN IVVRGYAIGY GIGSPHAQTI KVDYKQRYYT IENLDPSSHY VITLKAFNNV GEGIPLYESA VTRPHTDTSE VDLFVINAPY TPVPDPTPMM PPVGVQASIL SHDTIRITWA DNSLPKHQKI TDSRYYTVRW KTNIPANTKY KNANATTLSY LVTGLKPNTL YEFSVMVTKG RRSSTWSMTA HGATFELVPT SPPKDVTVVS KEGKPRTIIV NWQPPSEANG KITGYIIYYS TDVNAEIHDW VIEPVVGNRL THQIQELTLD TPYYFKIQAR NSKGMGPMSE AVQFRTPKAD SSDKMPNDQA LGSAGKGSRL PDLGSDYKPP MSGSNSPHGS PTSPLDSNML LVIIVSVGVI TIVVVVVIAV FCTRRTTSHQ KKKRAACKSV NGSHKYKGNC KDVKPPDLWI HHERLELKPI DKSPDPNPVM TDTPIPRNSQ DITPVDNSMD SNIHQRRNSY RGHESEDSMS TLAGRRGMRP KMMMPFDSQP PQPVISAHPI HSLDNPHHHF HSSSLASPAR SHLYHPSSPW PIGTSMSLSD RANSTESVRN TPSTDTMPAS SSQTCCTDHQ DPEGATSSSY LASSQEEDSG QSLPTAHVRP SHPLKSFAVP AIPPPGPPLY DPALPSTPLL SQQALEPSTF HSVKTASIGT LGRSRPPMPV VVPSAPEVQE TTRMLEDSES SYEPDELTKE MAHLEGLMKD LNAITTA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Neo1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: NEO1 (Neogenin 1 (NEO1))

Andere Bezeichnung: Neo1 (NEO1 Produkte)

Synonyme: cb333 Protein, zneo1 Protein, zgc:109795 Protein, 2610028H22Rik Protein, AI327052 Protein, D930014N22Rik Protein, Igdcc2 Protein, IGDCC2 Protein, NGN Protein, neogenin 1a Protein, neogenin 1 Protein, neogenin Protein, neo1a Protein, NEO1 Protein, LOC100448839 Protein, Neo1 Protein

Hintergrund: Multi-functional cell surface receptor regulating cell adhesion in many diverse developmental processes, including neural tube and mammary gland formation, myogenesis and angiogenesis. Receptor for members of the BMP, netrin, and repulsive guidance molecule (RGM) families. Netrin-Neogenin interactions result in a chemoattractive axon guidance response and cell-cell adhesion, the interaction between NEO1/Neogenin and RGMa and RGMb induces a chemorepulsive response. {ECO:0000269|PubMed:23744777}.

Molekulargewicht: 160.5 kDa Including tag.

UniProt: P97798

Pathways: Transition Metal Ion Homeostasis, Regulation of Muscle Cell Differentiation, Tube Formation