SLC7A10 Protein (AA 1-530) (Strep Tag)

Produktnummer ABIN3120843

Produktdetails

Target: SLC7A10 (Solute Carrier Family 7 (Neutral Amino Acid Transporter Light Chain, Asc System), Member 10 (SLC7A10))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-530

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SLC7A10 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequenz: MRRDSDMASH IQQPGGHGNP GPAPSPSPGP GPGPGASERV ALKKEIGLVS ACTIIIGNII GSGIFISPKG VLEHSGSVGL ALFVWVLGGG VTALGSLCYA ELGVAIPKSG GDYAYVTEIF GGLAGFLLLW SAVLIMYPTS LAVISMTFSN YVLQPVFPNC IPPATASRVL SMACLMLLTW VNSSSVRWAT RIQVIFTGGK LLALSLIITV GFVQIFQGHF EELRPTNAFA FWMTPSVGHL ALAFLQGSFA FSGWNFLNYV TEELVDPRKN LPRAIFISIP LVTFVYTFTN VAYFTAMSPQ ELLSSNAVAV TFGEKLLGYF SWVMPVSVAL STFGGINGYL FTSSRLCFSG AREGHLPSFL AMIHVRRCTP IPALLVCCGA TAVIMLVGDT YTLINYVSFI NYLCYGVTIL GLLVLRWRRP ALHRPIKVNL LVPVVYLVFW AFLLVFSFIS EPMVCGVGII IILTGVPIFF LGVFWRSKPK CVHRFTESMT RWGQELCFVV YPQGSLEEEE NGPMGQPSPL PITDKPLKTQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SLC7A10 (Solute Carrier Family 7 (Neutral Amino Acid Transporter Light Chain, Asc System), Member 10 (SLC7A10))

Andere Bezeichnung: Slc7a10 (SLC7A10 Produkte)

Synonyme: AL024237 Protein, Asc-1 Protein, D7Bwg0847e Protein, HASC-1 Protein, asc-1 Protein, solute carrier family 7 (cationic amino acid transporter, y+ system), member 10 Protein, solute carrier family 7 member 10 Protein, Slc7a10 Protein, SLC7A10 Protein

Hintergrund: Asc-type amino acid transporter 1 (Asc-1) (D-serine transporter) (Solute carrier family 7 member 10),FUNCTION: Associates with SLC3A2/4F2hc to form a functional heterodimeric complex that translocates small neutral L- and D-amino acids across the plasma membrane. Preferentially mediates exchange transport, but can also operate via facilitated diffusion (PubMed:10734121) (By similarity). Acts as a major transporter for glycine, L- and D-serine in the central nervous system. At the spinal cord and brainstem regulates glycine metabolism and glycinergic inhibitory neurotransmission by providing for glycine de novo synthesis from L-serine and glycine recycling from astrocytes to glycinergic motor neurons (PubMed:25755256, PubMed:27759100). At Schaffer collateral-CA1 synapses mediates D-serine and glycine release that modulates post-synaptic activation of NMDA receptors and excitatory glutamatergic transmission (By similarity). May regulate D-serine release from mesenchymal progenitors located in developing subcutaneous adipose tissue, favoring white adipocyte over thermogenic beige adipocyte lineage commitment (PubMed:33707431). {ECO:0000250|UniProtKB:P63116, ECO:0000269|PubMed:10734121, ECO:0000269|PubMed:25755256, ECO:0000269|PubMed:27759100, ECO:0000269|PubMed:33707431}.

Molekulargewicht: 57.5 kDa

UniProt: P63115