SLC4A2 Protein (AA 1-1237) (Strep Tag)

Produktnummer ABIN3132958

Produktdetails

Target: SLC4A2 (Solute Carrier Family 4, Anion Exchanger, Member 2 (erythrocyte Membrane Protein Band 3-Like 1) (SLC4A2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1237

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SLC4A2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MSSAPRRPAS GADSLHTPEP ESLSPGTPGF PEQEEDELRT LGVERFEEIL QEAGSRGGEE PGRSYGEEDF EYHRQSSHHI HHPLSTHLPP DARRRKTPQG PGRKPRRRPG ASPTGETPTI EEGEEDEEEA SEAEGFRAPP QQPSPATTPS AVQFFLQEDE GAERKPERTS PSPPTQTPHQ EAAPRASKGA QTGTLVEEMV AVASATAGGD DGGAAGRPLT KAQPGHRSYN LQERRRIGSM TGVEQALLPR VPTDESEAQT LATADLDLMK SHRFEDVPGV RRHLVRKNAK GSTQAAREGR EPGPTPRARP RAPHKPHEVF VELNELLLDK NQEPQWRETA RWIKFEEDVE EETERWGKPH VASLSFRSLL ELRRTLAHGA VLLDLDQQTL PGVAHQVVEQ MVISDQIKAE DRANVLRALL LKHSHPSDEK EFSFPRNISA GSLGSLLGHH HAQGTESDPH VTEPLIGGVP ETRLEVDRER ELPPPAPPAG ITRSKSKHEL KLLEKIPENA EATVVLVGCV EFLSRPTMAF VRLREAVELD AVLEVPVPVR FLFLLLGPSS ANMDYHEIGR SISTLMSDKQ FHEAAYLADE RDDLLTAINA FLDCSVVLPP SEVQGEELLR SVAHFQRQML KKREEQGRLL PPGAGLEPKS AQDKALLQMV EVAGAAEDDP LRRTGRPFGG LIRDVRRRYP HYLSDFRDAL DPQCLAAVIF IYFAALSPAI TFGGLLGEKT KDLIGVSELI MSTALQGVVF CLLGAQPLLV IGFSGPLLVF EEAFFSFCSS NELEYLVGRV WIGFWLVFLA LLMVALEGSF LVRFVSRFTQ EIFAFLISLI FIYETFYKLI KIFQEHPLHG CSGSNDSEAG SSSSSNMTWA TTILVPDNSS ASGQSGQEKP RGQPNTALLS LVLMAGTFFI AFFLRKFKNS RFFPGRIRRV IGDFGVPIAI LIMVLVDYSI EDTYTQKLSV PSGFSVTAPD KRGWVINPLG EKTPFPVWMM VASLLPAVLV FILIFMETQI TTLIISKKER MLQKGSGFHL DLLLIVAMGG ICALFGLPWL AAATVRSVTH ANALTVMSKA VAPGDKPKIQ EVKEQRVTGL LVALLVGLSM VIGDLLRQIP LAVLFGIFLY MGVTSLNGIQ FYERLHLLLM PPKHHPDVTY VKKVRTMRMH LFTALQLLCL ALLWAVMSTA ASLAFPFILI LTVPLRMVVL TRIFTEREMK CLDANEAEPV FDECEGVDEY NEMPMPV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SLC4A2 (Solute Carrier Family 4, Anion Exchanger, Member 2 (erythrocyte Membrane Protein Band 3-Like 1) (SLC4A2))

Andere Bezeichnung: Slc4a2 (SLC4A2 Produkte)

Synonyme: AE2 Protein, BND3L Protein, EPB3L1 Protein, HKB3 Protein, NBND3 Protein, SLC4A2 Protein, B3RP Protein, Ae2 Protein, Aep2 Protein, AE 2 Protein, Anion exchanger 2 Protein, solute carrier family 4 member 2 Protein, anion exchange protein 2 Protein, solute carrier family 4 (anion exchanger), member 2 Protein, SLC4A2 Protein, LOC100599599 Protein, Slc4a2 Protein

Hintergrund: Anion exchange protein 2 (AE 2) (Anion exchanger 2) (Band 3-related protein) (B3RP) (Non-erythroid band 3-like protein) (Solute carrier family 4 member 2),FUNCTION: Sodium-independent anion exchanger which mediates the electroneutral exchange of chloride for bicarbonate ions across the cell membrane (PubMed:2371270, PubMed:12958022, PubMed:18971331, PubMed:23341620, PubMed:24515893). Plays an important role in osteoclast differentiation and function (PubMed:18971331, PubMed:23341620). Regulates bone resorption and calpain-dependent actin cytoskeleton organization in osteoclasts via anion exchange-dependent control of pH (PubMed:23341620). Essential for intracellular pH regulation in CD8(+) T-cells upon CD3 stimulation, modulating CD8(+) T-cell responses (PubMed:24515893). {ECO:0000269|PubMed:12958022, ECO:0000269|PubMed:18971331, ECO:0000269|PubMed:23341620, ECO:0000269|PubMed:2371270, ECO:0000269|PubMed:24515893}., FUNCTION: [Isoform A]: Plays a critical role in male fertility and spermiogenesis. {ECO:0000269|PubMed:14673081}., FUNCTION: [Isoform B1]: Plays a critical role in male fertility and spermiogenesis. {ECO:0000269|PubMed:14673081}., FUNCTION: [Isoform B2]: Plays a critical role in male fertility and spermiogenesis. {ECO:0000269|PubMed:14673081}.

Molekulargewicht: 136.8 kDa

UniProt: P13808