CNTNAP1 Protein (AA 21-1385) (rho-1D4 tag)

Produktnummer ABIN3131836

Produktdetails

Target: CNTNAP1 (Contactin Associated Protein 1 (CNTNAP1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 21-1385

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses CNTNAP1 Protein ist gelabelt mit rho-1D4 tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: WGYYGCNEEL VGPLYARSLG ASSYYGLFTT ARFARLHGIS GWSPRIGDPN PWLQIDLMKK HRIRAVATQG AFNSWDWVTR YMLLYGDRVD SWTPFYQKGH NATFFGNVND SAVVRHDLHY HFTARYIRIV PLAWNPRGKI GLRLGIYGCP YTSSILYFDG DDAISYRFQR GASQSLWDVF AFSFKTEEKD GLLLHTEGSQ GDYVTLELQG AHLLLHMSLG SSPIQPRPGH TTVSLGGVLN DLSWHYVRVD RYGRDANFTL DGYAHHFVLN GDFERLNLEN EIFIGGLVGA ARKNLAYRHN FRGCIENVIY NRINIAEMAV MRHSRITFEG NVAFRCLDPV PHPINFGGPH NFVQVPGFPR RGRLAVSFRF RTWDLTGLLL FSHLGDGLGH VELMLSEGQV NVSIAQTGRK KLQFAAGYRL NDGFWHEVNF VAQENHAVIS IDDVEGAEVR VSYPLLIRTG TSYFFGGCPK PASRWGCHSN QTAFHGCMEL LKVDGQLVNL TLVEFRKLGY FAEVLFDTCG ITDRCSPNMC EHDGRCYQSW DDFICYCELT GYKGVTCHEP LYKESCEAYR LSGKYSGNYT IDPDGSGPLK PFVVYCDIRE NRAWTVVRHD RLWTTRVTGS SMDRPFLGAI QYWNASWEEV SALANASQHC EQWIEFSCYN SRLLNTAGGY PYSFWIGRNE EQHFYWGGSQ PGIQRCACGL DQSCVDPALH CNCDADQPQW RTDKGLLTFV DHLPVTQVVV GDTNRSNSEA QFFLRPLRCY GDRNSWNTIS FHTGAALRFP PIRANHSLDV SFYFRTSAPS GVFLENMGGP FCRWRRPYVR VELNTSRDVV FAFDIGNGDE NLTVHSDDFE FNDDEWHLVR AEINVKQARL RVDHRPWVLR PMPLQTYIWL VYDQPLYVGS AELKRRPFVG CLRAMRLNGV TLNLEGRANA SEGTFPNCTG HCTHPRFPCF HGGRCVERYS YYTCDCDLTA FDGPYCNHDI GGFFETGTWM RYNLQSALRS AAREFSHMLS RPVPGYEPGY VPGYDTPGYV PGYHGPGYRL PEYPRPGRPV PGYRGPVYNV TGEEVSFSFS TNSAPAVLLY VSSFVRDYMA VLIKEDGTLQ LRYQLGTSPY VYQLTTRPVT DGQPHSVNIT RVYRNLFIQV DYFPLTEQKF SLLVDSQLDS PKALYLGRVM ETGVIDPEIQ RYNTPGFSGC LSGVRFNNVA PLKTHFRTPR PMTAELAEAM RVQGELSESN CGAMPRLVSE VPPELDPWYL PPDFPYYHDD GWIAILLGFL VAFLLLGLVG MLVLFYLQNH RYKGSYHTNE PKATHDSHPG GKAPLPPSGP AQAPAPTPAP TQLPTPAPAP APAPASGPGP RDQNLPQILE ESRSE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Cntnap1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CNTNAP1 (Contactin Associated Protein 1 (CNTNAP1))

Andere Bezeichnung: Cntnap1 (CNTNAP1 Produkte)

Synonyme: CNTNAP1 Protein, CASPR Protein, CNTNAP Protein, NRXN4 Protein, P190 Protein, Caspr Protein, AI841080 Protein, NCP1 Protein, Nrxn4 Protein, p190 Protein, shm Protein, contactin associated protein 1 Protein, contactin associated protein-like 1 Protein, CNTNAP1 Protein, cntnap1 Protein, Cntnap1 Protein

Hintergrund: Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells. Mice that lack CNTAP1 exhibit tremor, ataxia, and significant motor paresis. Normal paranodal junctions fail to form, and the organization of the paranodal loops is disrupted. Contactin is undetectable in the paranodes, and potassium channels are displaced from the juxtaparanodal into the paranodal domains. Also results in a severe decrease in peripheral nerve conduction velocity.

Molekulargewicht: 155.4 kDa Including tag.

UniProt: O54991

Pathways: Cell-Cell Junction Organization