GPR137B Protein (AA 1-385) (Strep Tag)

Produktnummer ABIN3125087

Produktdetails

Target: GPR137B (G Protein-Coupled Receptor 137B (GPR137B))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-385

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses GPR137B Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MEAPPWEPVR NDSLPPTLSP AVPPYVKLGL TAVYTVFYAL LFVFIYAQLW LVLRYRHKRL SYQSVFLFLC LFWASLRTVL FSFYFRDFVA ANSFSPFVFW LLYCFPVCLQ FFTLTLMNLY FTQVIFKAKS KYSPELLKYR LPLYLASLFI SLVFLLVNLT CAVLVKTGDW DRKVIVSVRV AINDTLFVLC AISLSICLYK ISKMSLANIY LESKGSSVCQ VTAIGVTVIL LYTSRACYNL FILSFSQIKN VHSFDYDWYN VSDQADLKSQ LGDAGYVVFG VVLFVWELLP TTLVVYFFRV RNPTKDLTNP GMVPSHGFSP RSYFFDNPRR YDSDDDLAWN IAPQGLQGSF APDYCDWGQQ NNSFLAQAGT LHQDSTLDPD KASQG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: GPR137B (G Protein-Coupled Receptor 137B (GPR137B))

Andere Bezeichnung: Gpr137b (GPR137B Produkte)

Synonyme: TM7SF1 Protein, AI788917 Protein, AU016435 Protein, AW546472 Protein, C730041E01 Protein, C80550 Protein, C80741 Protein, D13Abb1e Protein, Tm7sf1 Protein, GPR137B Protein, gpr137ba Protein, tm7sf1a Protein, wu:fi39g02 Protein, zgc:101671 Protein, G protein-coupled receptor 137B Protein, G protein-coupled receptor 137B L homeolog Protein, G protein-coupled receptor 137c Protein, GPR137B Protein, gpr137b.L Protein, Gpr137b Protein, gpr137b Protein, gpr137c Protein

Hintergrund: Integral membrane protein GPR137B (Transmembrane 7 superfamily member 1 protein),FUNCTION: Lysosomal integral membrane protein that regulates the localization and activity of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Interacts with Rag GTPases and increases the lysosomial localization and activity of Rag GTPases and thereby regulates mTORC1 translocation and activity in lysosome. Involved in the regulation of lysosomal morphology and autophagy (By similarity). Acts also as a negative regulator of osteoclast activity (PubMed:31173907). Involved in interleukin-4-induced M2 macrophage polarization (PubMed:30595385). {ECO:0000250|UniProtKB:O60478, ECO:0000269|PubMed:30595385, ECO:0000269|PubMed:31173907}., FUNCTION: Acts also as a negative regulator of osteoclast activity (PubMed:31173907). Involved in interleukin-4-induced M2 macrophage polarization (PubMed:30595385). {ECO:0000269|PubMed:30595385, ECO:0000269|PubMed:31173907}.

Molekulargewicht: 44.1 kDa

UniProt: Q8BNQ3