GPR174 Protein (AA 1-335) (Strep Tag)

Produktnummer ABIN3121965

Produktdetails

Target: GPR174 (G Protein-Coupled Receptor 174 (GPR174))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-335

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses GPR174 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MTDNFTCNKT DGDNTDFRYF IYAVTYTVIL VPGLIGNILA LWVFYGYMKE TKRAVVFMIN LAIADLLQIL SLPLRIFYYL NHDWPFGPGL CMFCFYLKYV NMYASIYFLV CISVRRFWFL MYPFRFNDCK QKYDLYISII GWLIICLACL LFPLLRTNDD TPGNRTKCFV DLPIRNVNLA QSVAMITIGE VVGFVTPLMI VLYCTWKTAL SLQNKYPISQ HLGEKKKALK MILTCAGVFL VCFVPYHFSF PLDFLVKSNE IKSCFARRVI LIFHSVALCL ASLNSCLDPV IYYFTTNEFR RRLSRQDLPD NIQLHTKSYK IASNHATSTV AAELC
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: GPR174 (G Protein-Coupled Receptor 174 (GPR174))

Andere Bezeichnung: Gpr174 (GPR174 Produkte)

Synonyme: RGD1564689 Protein, GPR174 Protein, GPCR17 Protein, Gm376 Protein, G protein-coupled receptor 174 Protein, Gpr174 Protein, GPR174 Protein

Hintergrund: Probable G-protein coupled receptor 174,FUNCTION: G-protein-coupled receptor of lysophosphatidylserine (LysoPS) that plays different roles in immune response. Plays a negative role in regulatory T-cell accumulation and homeostasis (PubMed:26077720). Under inflammatory conditions where LysoPS production increases, contributes to the down-regulation of regulatory T-cell activity to favor effector response (PubMed:26077720). Mediates the suppression of IL-2 production in activated T-lymphocytes leading to inhibition of growth, proliferation and differentiation of T-cells (PubMed:29457279). Mechanistically, acts via G(12)/G(13)-containing heterotrimeric G proteins to trigger elevated cyclic AMP levels and protein kinase A/PKA activity, which may in turn act to antagonize proximal TCR signaling (PubMed:29457279). Plays an important role in the initial period of sepsis through the regulation of macrophage polarization and pro- and anti-inflammatory cytokine secretions (PubMed:30850582). Upon testosterone treatment, acts as a receptor for CCL21 and subsequently triggers through G(q)-alpha and G(12)/G(13) proteins a calcium flux leading to chemotactic effects on activated B-cells (PubMed:31875850). Signals via GNA13 and PKA to promote CD86 up-regulation by follicular B-cells (PubMed:35639700). {ECO:0000269|PubMed:26077720, ECO:0000269|PubMed:29457279, ECO:0000269|PubMed:30850582, ECO:0000269|PubMed:31875850, ECO:0000269|PubMed:35639700}.

Molekulargewicht: 38.8 kDa

UniProt: Q3U507