GPC1 Protein (AA 24-529) (His tag)

Produktnummer ABIN3137409

Produktdetails

Target: GPC1 (Glypican 1 (GPC1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 24-529

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses GPC1 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: DPASKSRSCS EVRQIYGAKG FSLSDVPQAE ISGEHLRICP QGYTCCTSEM EENLANHSRM ELESALHDSS RALQATLATQ LHGIDDHFQR LLNDSERTLQ EAFPGAFGDL YTQNTRAFRD LYAELRLYYR GANLHLEETL AEFWARLLER LFKQLHPQLL PDDYLDCLGK QAEALRPFGD APRELRLRAT RAFVAARSFV QGLGVASDVV RKVAQVPLAP ECSRAIMKLV YCAHCRGVPG ARPCPDYCRN VLKGCLANQA DLDAEWRNLL DSMVLITDKF WGPSGAESVI GGVHVWLAEA INALQDNKDT LTAKVIQACG NPKVNPHGSG PEEKRRRGKL ALQEKPSTGT LEKLVSEAKA QLRDIQDFWI SLPGTLCSEK MAMSPASDDR CWNGISKGRY LPEVMGDGLA NQINNPEVEV DITKPDMTIR QQIMQLKIMT NRLRGAYGGN DVDFQDASDD GSGSGSGGGC PDDTCGRRVS KKSSSSRTPL THALPGLSEQ EGQKTS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Gpc1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: GPC1 (Glypican 1 (GPC1))

Andere Bezeichnung: Gpc1 (GPC1 Produkte)

Synonyme: gpc1 Protein, zgc:63947 Protein, GPC1 Protein, Glypican-1 Protein, glypican Protein, AI462976 Protein, HSPG M12 Protein, HSPGM12 Protein, glypican 1b Protein, glypican 1 Protein, gpc1b Protein, GPC1 Protein, gpc1 Protein, Gpc1 Protein

Hintergrund: Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. Binds Cu(2+) or Zn(2+) ions. {ECO:0000250, ECO:0000269|PubMed:19732411, ECO:0000269|PubMed:19936054, ECO:0000269|PubMed:20100867, ECO:0000269|PubMed:21996748}.

Molekulargewicht: 56.6 kDa Including tag.

UniProt: Q9QZF2

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation