TDRD12 Protein (AA 1-1215) (Strep Tag)

Produktnummer ABIN3137048

Produktdetails

Target: TDRD12 (Tudor Domain Containing 12 (TDRD12))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1215

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses TDRD12 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MFEVLVLKIE DPGCFWVIIK GCSHFLEQEV DYQKLNTAMN DFYNSMCQDV EMKPLMLEEG QVCVVYCQEL KCWCRALIKS IISSADHYLA ECFLVDFAKY IPVKSKNIRV AVESFMQLPY RAKKFRLYGT KPVTLHIDFC EDNAEIVPAT KWDSAAIQYF QNLLRATTQV EAKLCAVEED TFEVYLYATI KNEKVCVNDD LVAKNFAYYV SPMGNKNLNP LEKPRQSLNS VTCSSKLSPS LTLWPMLLQG KDYHRMENKA LNYKDSLTDS PKMMLEKQQQ SLPLKHTEKC TESSVYWPTK RGITIYADPD VPSVSGSSQR PNEKPLRLTE KKDCDEKNGC VKLLQFLNPD PLRADGTSDL HQLQKVKLGT LQPGVVLRNR IEPCLTLEKS PLSADLKKMA LTCLNSDTED MKVAMEEMIP PEALQRNKFP GPSHTASYSW PPIARGCDMV VISHCGNDPL LYLPPLLTIL QMGGCYKSLP SRNGPLAVIV CPGWKKAQFI FELLGDYSMS SRPLHPVLLT IGLHKDEAKN MKLPRGCDVI VTTPHSLLRL LTYRSLLFLR LCHLVLDEVH MLFFEANEQM FAILDNFKKN VEVEERESAP HQIVAVGVHW NKHIDHLVRE FMKDPCVVIT ALEEAALYGS VQQVVHLCLE CEKTSTLLQV LDFVPSQAQK TLIFTCSVAE TETVCKGSPA EQGDKKTKSV LLLTERNASH AVGILRYLER ADAKIPSELY EFTAGVLEAK EDKKARRPLC PYLKAFGFCK DKRICPDRHH INPEMDIPRK LSNKTLPVFG HIRVIPFYIS NATNYFGRII DKHVDLYETL NAEMNEYFKE SSNKTSAEKV ENLGLYGLEE KTLFQRVQVL EVSQKEDTWG LGSILVKLID EGRTKLITRD QLLLLPEKFH TLPPQAVEFI VCRVKPADSE IEWNPKVTRY IHHKIVGKMH DAKVVLALGN TLWIDPMVHV TKLSNLKTSI IDYNVRAEIL SMGMGIDNSE HLEQLKKLYK EAKLPAFEDL PCQTSIPTTV EDTVCLQGTQ QGDGGTERGA GSQEDSDNQK PGVLSEDIGS ETISSAPQPH GRSFHPQIKW FQKDDVVILK IKIRNVKDYK CKFFTDRVIF SAWVGDKFYL ADLELQGDIR KDDCKCIIKD DEPLITLAKE KQECWCGLLK QRNPNVAFDF DHWEECEEDS PFSKVVNSKN LSCKVAALAE SSGSSSDTTD GSESE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TDRD12 (Tudor Domain Containing 12 (TDRD12))

Andere Bezeichnung: Tdrd12 (TDRD12 Produkte)

Synonyme: ECAT8 Protein, 2410004F06Rik Protein, 2410070K17Rik Protein, EG434165 Protein, tudor domain containing 12 Protein, TDRD12 Protein, Tdrd12 Protein

Hintergrund: Putative ATP-dependent RNA helicase TDRD12 (EC 3.6.4.13) (ES cell-associated transcript 8 protein) (Tudor domain-containing protein 12),FUNCTION: Probable ATP-binding RNA helicase required during spermatogenesis to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:24067652). Involved in the secondary piRNAs metabolic process (PubMed:24067652). Acts via the PET complex, a multiprotein complex required during the secondary piRNAs metabolic process for the PIWIL2 slicing-triggered loading of PIWIL4 piRNAs (PubMed:26669262). {ECO:0000269|PubMed:24067652, ECO:0000269|PubMed:26669262}.

Molekulargewicht: 137.6 kDa

UniProt: Q9CWU0