Regulating Synaptic Membrane Exocytosis 1 (RIMS1) (AA 1-1463) protein (Strep Tag)

Produktnummer ABIN3136988

Produktdetails

Target: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1463

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Strep Tag

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MSSAVGPRGP RPPTVPPPMQ ELPDLSHLTE EERNIIMAVM DRQKEEEEKE EAMLKCVVRD MAKPAACKTP RNAESQPHQP PLNIFRCVCV PRKPSSEEGG PDRNWRLHQQ FESYKEQVRK IGEEARRYQG EHKDDAPTCG ICHKTKFADG CGHLCSYCRT KFCARCGGRV SLRSNNEDKV VMWVCNLCRK QQEILTKSGA WFFGSGPQQP SQDGTLSDTA TGAGSEVPRE KKARLQERSR SQTPLSTAAV SSQDTASHGA PLDRNKGAEP SQQALGPEQK QASRSRSEPP RERKKAPGLS EQNGKGGQKS ERKRVPKSVV QPGEGTADER ERKERRETRR LEKGRSQDYP DRLEKREDGR VAEDEKQRKE EEGVSTPEYT SCEDVELESE SVSEKGDLDY WLDPATWHSR ETSPISSHPV TWQPSKEGDR LIGRVILNKR TTMPKESGAL LGLKVVGGKM TDLGRLGAFI TKVKKGSLAD VVGHLRAGDE VLEWNGKPLP GATNEEVYNI ILESKSEPQV EIIVSRPIGD IPRIPESSHP PLESSSSSFE SQKMERPSIS VISPTSPGAL KDAPQVLPGQ LSVKLWYDKV GHQLIVNVLQ ATDLPPRVDG RPRNPYVKMY FLPDRSDKSK RRTKTVKKLL EPKWNQTFVY SHVHRRDFRE RMLEITVWDQ PRVQDEESEF LGEILIELET ALLDDEPHWY KLQTHDESSL PLPQPSPFMP RRHIHGESSS KKLQRSQRIS DSDISDYEVD DGIGVVPPVG YRASARESKA TTLTVPEQQR TTHHRSRSVS PHRGDDQGRP RSRLPNVPLQ RSLDEIHPTR RSRSPTRHHD ASRSLADHRS RHAESQYSSE PDSELLMLPR AKRGRSAECL HMTSELQPSL DRARSASTNC LRPDTSLHSP ERERGRWSPS LARRRPASPR IQIQHASPEN DRHSRKSERS SIQKQSRKGT ASDADRVLPP CLSRRGYAIP RATDQPVIRG KHTTRSRSSE HSSIRTLCSM HHLAPGGSAP PSPLLTRTHR QGSPTQSPPA DTSFGSRRGR QLPQVPVRSG SIEQASLVVE ERTRQMKMKV HRFKQTTGSG SSQELDHEQY SKYNIHKDQY RSCDNASAKS SDSDVSDVSA ISRASSTSRL SSTSFMSEQS ERPRGRISSF TPKMQGRRMG TSGRAIIKST SVSGEIYTLE HNDGSQSDTA VGTVGAGGKK RRSSLSAKVV AIVSRRSRST SQLSQTESGH KKLKSTIQRS TETGMAAEMR KMVRQPSRES TDGSINSYSS EGNLIFPGVR VGPDSQFSDF LDGLGPAQLV GRQTLATPAM GDIQIGMEDK KGQLEVEVIR ARSLTQKPGS KSTPAPYVKV YLLENGACIA KKKTRIARKT LDPLYQQSLV FDESPQGKVL QVIVWGDYGR MDHKCFMGVA QILLEELDLS SMVIGWYKLF PPSSLVDPTL TPLTRRASQS SLESSSGPPC IRS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Andere Bezeichnung: Rims1 (RIMS1 Produkte)

Synonyme: RIMS1 Protein, MGC121671 Protein, C030033M19Rik Protein, RIM1 Protein, RIM1a Protein, RIM1alpha Protein, Rab3ip1 Protein, Rim Protein, Serg1 Protein, mKIAA0340 Protein, CORD7 Protein, RAB3IP2 Protein, RIM Protein, Rim1 Protein, regulating synaptic membrane exocytosis 1 Protein, RIMS1 Protein, rims1 Protein, Rims1 Protein

Hintergrund: Regulating synaptic membrane exocytosis protein 1 (Rab-3-interacting molecule 1) (RIM 1) (Rab-3-interacting protein 1),FUNCTION: Rab effector involved in exocytosis (PubMed:11797009). May act as scaffold protein that regulates neurotransmitter release at the active zone. Essential for maintaining normal probability of neurotransmitter release and for regulating release during short-term synaptic plasticity (PubMed:11797009). Plays a role in dendrite formation by melanocytes (By similarity). {ECO:0000250|UniProtKB:Q86UR5, ECO:0000269|PubMed:11797009}.

Molekulargewicht: 163.2 kDa

UniProt: Q99NE5

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity