CACNA1D Protein (AA 1485-2179) (His tag)
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- Target Alle CACNA1D Proteine anzeigen
- CACNA1D (Calcium Channel, Voltage-Dependent, L Type, alpha 1D Subunit (CACNA1D))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1485-2179
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Spezies
- Maus
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Quelle
- Insektenzellen
- Aufreinigungstag / Konjugat
- Dieses CACNA1D Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
- Sequenz
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DNFDYLTRDW SILGPHHLDE FKRIWSEYDP EAKGRIKHLD VVTLLRRIQP PLGFGKLCPH RVACKRLVAM NMPLNSDGTV MFNATLFALV RTALKIKTEG NLEQANEELR AVIKKIWKKT SMKLLDQVVP PAGDDEVTVG KFYATFLIQD YFRKFKKRKE QGLVGKYPAK NTTIALQAGL RTLHDIGPEI RRAISCDLQD DEPEDSKPEE EDVFKRNGAL LGNHVNHVNS DRRDSLQQTN TTHRPLHVQR PSMPPASDTE KPLFPPAGNS GCHNHHNHNS IGKQAPTSTN ANLNNANMSK AAHGKPPSIG NLEHVSENGH YSCKHDRELQ RRSSIKRTRY YETYIRSESG DEQFPTICRE DPEIHGYFRD PRCLGEQEYF SSEECCEDDS SPTWSRQNYN YYNRYPGSSM DFERPRGYHH PQGFLEDDDS PTGYDSRRSP RRRLLPPTPP SHRRSSFNFE CLRRQSSQDD VLPSPALPHR AALPLHLMQQ QIMAVAGLDS SKAQKYSPSH STRSWATPPA TPPYRDWSPC YTPLIQVDRS ESMDQVNGSL PSLHRSSWYT DEPDISYRTF TPASLTVPSS FRNKNSDKQR SADSLVEAVL ISEGLGRYAR DPKFVSATKH EIADACDLTI DEMESAASTL LNGSVCPRAN GDMGPISHRQ DYELQDFGPG YSDEEPDPGR EEEDLADEMI CITTL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Cacna1d Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Protein is endotoxin free.
- Güteklasse
- Crystallography grade
- Top Product
- Discover our top product CACNA1D Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Kommentare
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- CACNA1D (Calcium Channel, Voltage-Dependent, L Type, alpha 1D Subunit (CACNA1D))
- Andere Bezeichnung
- Cacna1d (CACNA1D Produkte)
- Synonyme
- cacna1d Protein, cav1.3a Protein, isl Protein, si:dz136d19.3 Protein, si:dz223c6.1 Protein, ca(v)1.3 Protein, cav1.3 Protein, CACH3 Protein, CACN4 Protein, CACNL1A2 Protein, CCHL1A2 Protein, Cav1.3 Protein, SANDD Protein, 8430418G19Rik Protein, C79217 Protein, Cacnl1a2 Protein, Cchl1a Protein, Cchl1a2 Protein, D-LTCC Protein, calcium channel, voltage-dependent, L type, alpha 1D subunit, a Protein, calcium voltage-gated channel subunit alpha1 D Protein, calcium channel, voltage-dependent, L type, alpha 1D subunit Protein, calcium channel, voltage-dependent, L type, alpha 1D subunit L homeolog Protein, cacna1da Protein, CACNA1D Protein, cacna1d Protein, cacna1d.L Protein, Cacna1d Protein
- Hintergrund
- Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). {ECO:0000269|PubMed:16354915}.
- Molekulargewicht
- 79.8 kDa Including tag.
- UniProt
- Q99246
- Pathways
- Sensory Perception of Sound
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