HECW1 Protein (AA 1-1604) (Strep Tag)

Produktnummer ABIN3136612

Produktdetails

Target: HECW1 (HECT, C2 and WW Domain Containing E3 Ubiquitin Protein Ligase 1 (HECW1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1604

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses HECW1 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MLLHLCSVKN LYQNRFLGLA AMASPSRNSQ SRRRCKEPLR YSYNPDQFHN IDIRNGAHDA ITIPRSTSDT DLVTSDSRST LMVSSSYYSI GHSQDLVIHW DIKEEVDAGD WIGMYLIGEV SSENFLDYKN RGVNGSHRGQ IIWKIDASSY FVESETKICF KYYHGVSGAL RATTPSVTVK NSAAPIFKGI GSEETAQSQG SRRLISFSLS DFQAMGLKKG MFFNPDPYLK ISIQPGKHSI FPALPHHGQE RRSTIIGNTV NPIWQAEHFS FVSLPTDVLE IEVKDKFAKS RPIIKRFLGK LSMPVQRLLE RHAIGDRVVS YTLGRRLPTD HVSGQLQFRF EITSSIHADD EEISLSAEPE SSAETQDSIM NSMVGNSNGE PSGDATEFCK DAKPESPSEG NGVNSSENQN QEHAGPVEEA AGAMEARDGS NVSEAPEEPG ELQDPEQHDT QPTLSAEEVA EGLPLDEDSP SSLLPEENTA LGSKVEEETV PENGAREEEM QKGKDEEEEE EDVSTLEQGE PGLELRVSVR KKSRPCSLPV SELETVIASA CGDAETPRTH YIRIHTLLHS MPSAQRGSTT EEEDGLEEES TLKESSEKDG LSEVDTIAAD PQSMEDGESD GATLCMAPSD CSGGHFSSLS KGIGAGQDGE AHPSTGSESD SSPQQGADHS CEGCDASCCS PSCYSTSCYS SSCYSSSCYS SSCYNGNNRF ASHTRFSSVD SAKISESTVF SSQEDEEEEN SAFESVPDSV QSPELDPEST NGAGPWQDEL AAPGGNAARS TEGLESPMAG PSNRREGECP ILHNSQPISQ LPSLRPEHHH YPTIDEPLPP NWEARIDSHG RVFYVDHINR TTTWQRPSMA PTPDGMIRSG SVHQMEQLNR RYQNIQRTMA TERAEEDSGN QNSEQIPDGG GGGGGGSDSE AESSQSSLDL RREGSLSPVN SQKVTLLLQS PAVKFITNPE FFTVLHANYS AYRVFTSSTC LKHMILKVRR DARNFERYQH NRDLVNFINM FADTRLELPR GWEIKTDHQG KSFFVDHNSR ATTFIDPRIP LQNGRLPNHL THRQHLQRLR SYSAGEASEV SRNRGASLLA RPGHSLIAAI RSQHQHESLP LAYNDKIVAF LRQPNIFEML QERQPSLARN HTLREKIHYI RTEGNHGLDK LSCDADLVIL LSLFEEEIMS YVPLQSAFHP GYSFSPRCSP CSSPQNSPGL QRASARAPSP YRRDFEAKLR NFYRKLEAKG FGQGPGKIKL IIRRDHLLEG TFNQVMAYSR KELQRNKLYI TFVGEEGLDY SGPSREFFFL LSQELFNPYY GLFEYSANDT YTVQISPMSA FVENYLEWFR FSGRILGLAL IHQYLLDAFF TRPFYKGLLK LPCDLSDLEY LDEEFHQSLQ WMKDNNITDI LDLTFTVNEE VFGQVTEREL KSGGANTQVT EKNKKEYIER MVKWRVERGV VQQTEALLRG FYEVVDSRLV SVFDARELEL VIAGTAEIDL NDWRNNTEYR GGYHDGHLVI RWFWAAVERF NNEQRLRLLQ FVTGTSSVPY EGFAALRGSN GLRRFCIEKW GKITSLPRAH TCFNRLDLPP YPSYSMLYEK LLTAVEETST FGLE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: HECW1 (HECT, C2 and WW Domain Containing E3 Ubiquitin Protein Ligase 1 (HECW1))

Andere Bezeichnung: Hecw1 (HECW1 Produkte)

Synonyme: NEDL1 Protein, 9330116H24Rik Protein, AV273951 Protein, E130207I19Rik Protein, Nedl1 Protein, HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 Protein, HECW1 Protein, Hecw1 Protein

Hintergrund: E3 ubiquitin-protein ligase HECW1 (EC 2.3.2.26) (HECT, C2 and WW domain-containing protein 1) (HECT-type E3 ubiquitin transferase HECW1) (NEDD4-like E3 ubiquitin-protein ligase 1) (mNEDL1),FUNCTION: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1. {ECO:0000250, ECO:0000269|PubMed:14684739}.

Molekulargewicht: 179.5 kDa

UniProt: Q8K4P8