SULF1 Protein (AA 23-870) (His tag)

Produktnummer ABIN3136515

Produktdetails

Target: SULF1 (Sulfatase 1 (SULF1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 23-870

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses SULF1 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: STVRSQRFRG RIQQERKNIR PNIILVLTDD QDVELGSLQV MNKTRKIMEQ GGATFTNAFV TTPMCCPSRS SMLTGKYVHN HNVYTNNENC SSPSWQAMHE PRTFAVYLNN TGYRTAFFGK YLNEYNGSYI PPGWREWLGL IKNSRFYNYT VCRNGIKEKH GFDYAKDYFT DLITNESINY FKMSKRMYPH RPIMMVISHA APHGPEDSAP QFSKLYPNAS QHITPSYNYA PNMDKHWIMQ YTGPMLPIHM EFTNVLQRKR LQTLMSVDDS VERLYNMLVE SGELDNTYII YTADHGYHIG QFGLVKGKSM PYDFDIRVPF FIRGPSIEPG SIVPQIVLNI DLAPTILDIA GLDSPSDVDG KSVLKLLDLE KPGNRFRTNK KAKIWRDTFL VERGKFLRKK EESGKNIQQS NHLPKYERVK ELCQQARYQT ACEQPGQNWQ CIEDTSGKLR IHKCKGPSDL LTVRQNARNL YSRGLHDKDK ECHCRDSGYR SSRSQRKNQR QFLRNKGTPK YKPRFVHTRQ TRSLSVEFEG EIYDINLEEE ELQVLPPRSI AKRHDEGHQG FIGHQAAAGD IRNEMLADSN NAVGLPATVR VTHKCFILPN DTIHCERELY QSARAWKDHK AYIDKEIEVL QDKIKNLREV RGHLKKRKPE ECGCGDQSYY NKEKGVKRQE KLKSHLHPFK EAAAQEVDSK LQLFKEHRRR KKERKEKKRQ RKGEECSLPG LTCFTHDNNH WQTAPFWNLG SFCACTSSNN NTYWCLRTVN ETHNFLFCEF ATGFLEYFDM NTDPYQLTNT VHTVERSILN QLHIQLMELR SCQGYKQCNP RPKSLDIGAK EGGNYDPHRG QLWDGWEG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Sulf1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SULF1 (Sulfatase 1 (SULF1))

Andere Bezeichnung: Sulf1 (SULF1 Produkte)

Synonyme: HSULF-1 Protein, SULF-1 Protein, CG6725 Protein, DSulf Protein, DSulf1 Protein, Dmel\\CG6725 Protein, Dsulf1 Protein, Neu5 Protein, sulf1 Protein, AI467640 Protein, AW121680 Protein, mKIAA1077 Protein, fk14e08 Protein, wu:fk14e08 Protein, SULF1 Protein, sulf-1 Protein, XtSulf1 Protein, hsulf-1 Protein, XtSulf-1 Protein, sulfatase 1 Protein, Sulfated Protein, extracellular sulfatase SULF-1 homolog Protein, SULF1 Protein, Sulf1 Protein, sulf1 Protein, LOC410233 Protein

Hintergrund: Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Diminishes HSPG (heparan sulfate proteoglycans) sulfation, inhibits signaling by heparin-dependent growth factors, diminishes proliferation, and facilitates apoptosis in response to exogenous stimulation (By similarity). {ECO:0000250}.

Molekulargewicht: 99.5 kDa Including tag.

UniProt: Q8K007

Pathways: EGFR Signaling Pathway