NRIP1 Protein (AA 1-1161) (Strep Tag)

Produktnummer ABIN3136401

Produktdetails

Target: NRIP1 (Nuclear Receptor Interacting Protein 1 (NRIP1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1161

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses NRIP1 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequenz: MTHGEELGSD VHQDSIVLTY LEGLLMHQAA GGSGTAINKK SAGHKEEDQN FNLSGSAFPS CQSNGPTVST QTYQGSGMLH LKKARLLQSS EDWNAAKRKR LSDSIVNLNV KKEALLAGMV DSVPKGKQDS TLLASLLQSF SSRLQTVALS QQIRQSLKEQ GYALSHESLK VEKDLRCYGV ASSHLKTLLK KSKTKDQKSG PTLPDVTPNL IRDSFVESSH PAVGQSGTKV MSEPLSCAAR LQAVASMVEK RASPAASPKP SVACSQLALL LSSEAHLQQY SREHALKTQN AHQVASERLA AMARLQENGQ KDVGSSQLSK GVSGHLNGQA RALPASKLVA NKNNAATFQS PMGVVPSSPK NTSYKNSLER NNLKQAANNS LLLHLLKSQT IPTPMNGHSQ NERASSFESS TPTTIDEYSD NNPSFTDDSS GDESSYSNCV PIDLSCKHRI EKPEAERPVS LENLTQSLLN TWDPKIPGVD IKEDQDTSTN SKLNSHQKVT LLQLLLGHKS EETVERNASP QDIHSDGTKF SPQNYTRTSV IESPSTNRTT PVSTPPLYTA SQAESPINLS QHSLVIKWNS PPYACSTPAS KLTNTAPSHL MDLTKGKESQ AEKPAPSEGA QNSATFSASK LLQNLAQCGL QSSGPGEEQR PCKQLLSGNP DKPLGLIDRL NSPLLSNKTN AAEESKAFSS QPAGPEPGLP GCEIENLLER RTVLQLLLGN SSKGKNEKKE KTPARDEAPQ EHSERAANEQ ILMVKIKSEP CDDFQTHNTN LPLNHDAKSA PFLGVTPAIH RSTAALPVSE DFKSEPASPQ DFSFSKNGLL SRLLRQNQES YPADEQDKSH RNSELPTLES KNICMVPKKR KLYTEPLENP FKKMKNTAVD TANHHSGPEV LYGSLLHQEE LKFSRNELDY KYPAGHSSAS DGDHRSWARE SKSFNVLKQL LLSENCVRDL SPHRSDSVPD TKKKGHKNNA PGSKPEFGIS SLNGLMYSSP QPGSCVTDHR TFSYPGMVKT PLSPPFPEHL GCVGSRPEPG LLNGCSVPGE KGPIKWVIAD MDKNEYEKDS PRLTKTNPIL YYMLQKGGGN SVTTQETQDK DIWREPASAE SLSQVTVKEE LLPAAETKAS FFNLRSPYNS HMGNNASRPH STNGEVYGLL GNALTIKKES E
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: NRIP1 (Nuclear Receptor Interacting Protein 1 (NRIP1))

Andere Bezeichnung: Nrip1 (NRIP1 Produkte)

Synonyme: MGC68665 Protein, NRIP1 Protein, RIP140 Protein, 6030458L20Rik Protein, 8430438I05Rik Protein, 9630050P12 Protein, AA959574 Protein, AW456757 Protein, nuclear receptor interacting protein 1 S homeolog Protein, nuclear receptor interacting protein 1 Protein, circumsporozoite protein Protein, nrip1.S Protein, NRIP1 Protein, nrip1 Protein, Nrip1 Protein, LOC100627733 Protein

Hintergrund: Nuclear receptor-interacting protein 1 (Nuclear factor RIP140) (Receptor-interacting protein 140),FUNCTION: Modulates transcriptional repression by nuclear hormone receptors such as NR2C1, thyroid hormone receptor and retinoic acid receptor/RARA. Essential for cumulus expansion and follicle rupture during ovulation. Also controls the balance between fat accumulation and energy expenditure. Positive regulator of the circadian clock gene expression: stimulates transcription of BMAL1, CLOCK and CRY1 by acting as a coactivator for RORA and RORC. Involved in the regulation of ovarian function (PubMed:11100122). Plays a role in renal development (PubMed:28381549). {ECO:0000269|PubMed:10531331, ECO:0000269|PubMed:11100122, ECO:0000269|PubMed:15130509, ECO:0000269|PubMed:15155905, ECO:0000269|PubMed:15919748, ECO:0000269|PubMed:21628546, ECO:0000269|PubMed:28381549, ECO:0000269|PubMed:9774688}.

Molekulargewicht: 126.3 kDa

UniProt: Q8CBD1

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway