CCDC79 Protein (AA 1-768) (Strep Tag)

Produktnummer ABIN3136347

Produktdetails

Target: CCDC79 (Coiled-Coil Domain Containing 79 (CCDC79))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-768

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses CCDC79 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MESEKPKKTQ EMKTDLKLLL ECLKYHMGNP LSQKEVLITI HSVCKQNSDA GIYFREIGGL MFIINLAKSS EQSLVKEAAL YTLGSIAEEN VYCQQSLCTS ELFQDLTGLL TNDDSNTNLK RMSVYVLLVL VSNNRNGQTL VREVGCIEVL SQMFRTVLSN YELNLSDNSV FQSYLLWSSV CSTLCVCVNN PQNDENQMLC CSLFPCVNEW LMNCMRPEVI RPICSFIGLT LANNTHAQNC FVSSGGLDVL CQVLVQLESD SHNTLSSAKL AVIVTKTMDA CITDNSAAFT VVLSKYHIVS TLLALLLHES LDSREKFSII LAIGHCTEDC EKNQYELLKN NGLPLMIQAL TEFKNEDLSK AATYVLHNCK KITGKLSLSL GQNSFGENEI ELKDISEKET LREHWKAAKE ILCRIKQFEK GGKEEKQQNR SGHYKDNTPS MKVNIQTNLK RLCADSTGGT RAEDKDINQS RELRSYKPSE IMSKACANEN QLTTRKKNTN PVHPFCKEKG QSKIVHETTP SCAQNLDKEK TFDQKDSVSQ SSDQVLKHLP HTVKNRKQVP ETDPFTLCLD IIDREVGIQA TDSCSRMLKY TCSGCIVARK LLNSRNFSKF LHSCAYQCVH HKVIMEAEDK YKNELRKTFI CAKKILLTPC RRRQLCKEST ASEELKIVHQ KPDSKKLPGL EAQALNTSIP EAMERRSPVP GQSGLHKKRR IRKDFTKEEV NYLFHGVKTM GNHWNSILWS FPFQKGRRAV DLAHKYHRLI KGPSCAAL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CCDC79 (Coiled-Coil Domain Containing 79 (CCDC79))

Andere Bezeichnung: Terb1 (CCDC79 Produkte)

Synonyme: 4930532D21Rik Protein, BB085179 Protein, si:dkey-30c15.5 Protein, telomere repeat binding bouquet formation protein 1 Protein, TERB1 Protein, Terb1 Protein, terb1 Protein

Hintergrund: Telomere repeats-binding bouquet formation protein 1 (Coiled-coil domain-containing protein 79),FUNCTION: Meiosis-specific telomere-associated protein involved in meiotic telomere attachment to the nucleus inner membrane, a crucial step for homologous pairing and synapsis (PubMed:24885367, PubMed:24413433, PubMed:26548954). Component of the MAJIN-TERB1-TERB2 complex, which promotes telomere cap exchange by mediating attachment of telomeric DNA to the inner nuclear membrane and replacement of the protective cap of telomeric chromosomes: in early meiosis, the MAJIN-TERB1-TERB2 complex associates with telomeric DNA and the shelterin/telosome complex. During prophase, the complex matures and promotes release of the shelterin/telosome complex from telomeric DNA (PubMed:26548954). In the MAJIN-TERB1-TERB2 complex, TERB1 probably mediates association with the shelterin/telosome complex via interaction with TERF1, promoting priming telomeric DNA attachment' (PubMed:26548954). Promotes telomere association with the nuclear envelope and deposition of the SUN-KASH/LINC complex (PubMed:24885367, PubMed:24413433). Also recruits cohesin to telomeres to develop structural rigidity (PubMed:24413433). {ECO:0000269|PubMed:24413433, ECO:0000269|PubMed:26548954, ECO:0000305|PubMed:24885367}.

Molekulargewicht: 86.8 kDa

UniProt: Q8C0V1