LARS Protein (AA 1-1178) (Strep Tag)

Produktnummer ABIN3136235

Produktdetails

Target: LARS (Leucyl-tRNA Synthetase (LARS))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1178

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses LARS Protein ist gelabelt mit Strep Tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequenz: MAGRKGTAKV DFLKEIEKEA QQKWEAEKVF EVSASRLEKQ KQSSKGKYFV TFPYPYMNGR LHLGHTFSLS KCEFAVGYQR LKGKSCLFPF GLHCTGMPIK ACADKLKREI ELYGCPPDFP EEEEEEEESS AKPGDIVVRD KAKGKKSKAA AKAGSSKYQW DIMKSLGLSD DDIVKFSEAE HWLDYFPPLA VQDLKTIGLK VDWRRSFITT DVNPYYDSFV RWQFLTLRER NKIKFGKRYT IYSPKDGQPC MDHDRQTGEG VGPQEYTLVK LKVLEPYPSK LSGLKGKNIF LVAATLRPET MFGQTNCWVR PDMKYIGFET ANGDIFICTQ RAARNMSYQG FTKHNGVVPV VKELMGEEIL GASLSAPLTC YKVVYVLPML TIKEDKGTGV VTSVPSDSPD DLAALRDLKK KQALRTKFGI RDDMVLPFEP VPVLEIPGIG NLPAVTVCDE LKIQSQNDRE KLAEAKEKLY LRGFYDGVML VDGFKGQKIQ HVKKTIQKNM IDAGDALIYM EPEKQVMSRS ADECVVALCD QWYLDYGDEN WKKQTFQCLK NMETFCEESR KNFEASLDWL QEHACSRTYG LGTRLPWDEQ WLIESLSDST IYMAFYTVAH LLQGGDLNGQ AESPLGIRPQ QMTKDVWDYV FFKDAPFPKT QIPKEKLDQL KQEFEFWYPV DLRASGKDLI PNHLSYYIYN HVAMWPEQSD KWPVSVRANG HLLLNSEKMS KSTGNFLTLS QAVDKFSADG MRLALADAGD TVEDANFVEA MADAGILRLY TWVEWVKEML ASCSSLRSGP ADSFNDRVFA SEMNAGIIKT DQNYEKMMFK EALKTGFFEF QAAKDKYREL ATEGMHRELV FRFIEVQTIL LTPFCPHLCE HIWTLLGKPD SIMHASWPVA GPVDESLIRS SQYLMEVAHD LRLRLKNYMM PAKGKKTDKQ PAQRPSHCTI YVAKNYPVWQ HITLTTLRSH FEANNGKLPD NKVIASELGS LPELKKYMKK VMPFVAMIKE NMEKKGPRVL DLELEFDEQA VLMENIVYLT NSLELEHIEV KFASEAEDKV REECCPGKPL NVFRTEPGVP VSLVNPQPSS GHFSTKIDIR QGDSCESIIR RLMKTDRGIK DLSKVKLMRF DDPLLGPRRV PVLGREHSEK TLISENAVFH VDLVSKKVHL TENGLRTDIG DTMVYLVH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: LARS (Leucyl-tRNA Synthetase (LARS))

Andere Bezeichnung: Lars1 (LARS Produkte)

Synonyme: HSPC192 Protein, LARS1 Protein, LEURS Protein, LEUS Protein, LRS Protein, PIG44 Protein, RNTLS Protein, hr025Cl Protein, CG7479 Protein, Dmel\\CG7479 Protein, LeuRS Protein, F21M12.1 Protein, F21M12_1 Protein, BA4991 Protein, DDBDRAFT_0183834 Protein, DDBDRAFT_0231251 Protein, DDB_0183834 Protein, DDB_0231251 Protein, 2310045K21Rik Protein, 3110009L02Rik Protein, AW536573 Protein, mKIAA1352 Protein, leucyl-tRNA synthetase Protein, Leucyl-tRNA synthetase, mitochondrial Protein, ATP binding/leucine-tRNA ligases/aminoacyl-tRNA ligase Protein, leucine--tRNA ligase Protein, leucyl-tRNA synthetase LeuS Protein, leucyl-tRNA ligase Protein, leucine-tRNA ligase Protein, LARS Protein, LeuRS-m Protein, AT1G09620 Protein, leuS Protein, APH_RS02290 Protein, mleuS Protein, Lars Protein

Hintergrund: Leucine--tRNA ligase, cytoplasmic (EC 6.1.1.4) (Leucyl-tRNA synthetase) (LeuRS),FUNCTION: Aminoacyl-tRNA synthetase that catalyzes the specific attachment of leucine to its cognate tRNA (tRNA(Leu)). It performs tRNA aminoacylation in a two-step reaction: Leu is initially activated by ATP to form a leucyl-adenylate (Leu-AMP) intermediate, then the leucyl moiety is transferred to the acceptor 3' end of the tRNA to yield leucyl-tRNA. To improve the fidelity of catalytic reactions, it is also able to hydrolyze misactivated aminoacyl-adenylate intermediates (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing). {ECO:0000250|UniProtKB:Q9P2J5}.

Molekulargewicht: 134.2 kDa

UniProt: Q8BMJ2

Pathways: EGFR Signaling Pathway