MADD Protein (AA 2-1577) (His tag)

Produktnummer ABIN3136009

Produktdetails

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-1577

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses MADD Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA

Sequenz: VQKKFCPRLL DYLVIVGARH PSSDSVAQTP ELLRRYPLED HPEFPLPPDV VFFCQPEGCL SVRQRRMSLR DDTSFVFTLT DKDTGVTRYG ICVNFYRSFQ KRMPKEKVEG GAGPRGKEGA HTSGASEEAA TGSSESGSTL QPPSADSTPD INQSPWGKRR AKAGSRSRNS TLTSLCVLSH YPFFSTFREC LYTLKRLVDC CSERLLGKKL GIPRGVQRDT MWRIFTGSLL VEEKSSALLQ DLREIEAWIY RLLRSPVPVS GQKRVDIEVL PQELQQALTF ALPDPSRFTL VDFPLHLPLE LLGVDACLQV LTCILLEHKV VLQSRDYNAL SMSVMAFVAM IYPLEYMFPV IPLLPTCMAS AEQLLLAPTP YIIGVPASFF LYKLDFKMPD DVWLVDLDSN RVIAPTNAEV LPILPEPESL ELKKHLKQAL ASMSLNTQPI LNLEKFHEGQ EIPLLLGRPS NDLQSTPSTE FNPLIYGNDV DSVDVATRVA MVRFFNSANV LQGFQMHTRT LRLFPRPVVA FQAGSFLASR PRQTPFAEKL ARTQAVEYFG EWILNPSNYA FQRIHNNTFD PALIGDKPKW YAHQLQPIHY RVYDGNSQLA EALSVPPERD SDSDPTEDSG SDSQDYDDSS SSYSSLGDFV SEMMKCDING DTPNVDPLTH AALGDASEVE IDELQPQKEG EEPGPDSENS QENPPLRSSS STTASSSPST VVHSAHSEAA DSTEMGDKAT AGISKPLPPV PPSICKSTVD RRQTETGEGS VCQRTYDNPY FEPQYGFPPE EDEEEQGESY TPRFSQHVSG SRAQKLLRPN SLKLASDSDA ESDSRASSPN STVSNNSTEG FGGIMSFASS LYRNHSTSFS LSNLTLPTKG AREKTTPFPS LKVFGLNTLM EIVTEAGPGS GEGNRRALVD QKSSVIKHSP TVKREPSSPQ GRSSNSSENQ QFLKEVVHSV LDGQGVGWLN MKKVRRLLES EQLRVFVLSK LNRAVQSEDD ARQDVIQDVE ISRKVYKGML DLLKCTVLSL EQSYAHAGLG GMASIFGLLE IAQTHYYSKE PDKRKRSPTE NVNTPVGKDP GLAGRGDPKA MAQLRVPQLG PRAPSATGKG PKELDTRSLK EENFVASVEL WNKHQEVKKQ KALEKQRPEG IKPVFDLGET EEKKSQMSAD SGVSLTSASQ RTDQDSVIGV SPAVMIRSSS QDSEVSTVSN SSGETLGADS DLSSNAGDGP GGEGSAHLAS SRATLSDSEI ETNSATSAIF GKAHSLKPKE KPAGSPIRSS EDVSQRVYLY EGLLGRDKGS MWDQLEDAAM ETFSLSKERS TLWDQMQFWE DAFLDAVMLE REGMGMDQGP QEMIDRYLSL GEHDRKRLED DEDRLLATLL HNLISYMLLM KVNKNDIRKK VRRLMGKSHI GLVYSQQVNE VLDQLNSLNG RDLSIRSSGS RHMKKQTFVV HAGTDTNGDI FFMEVCDDCV VLRSNIGTVY ERWWYEKLIN MTYCPKTKVL CLWRRNGSET QLNKFYTKKC RELYYCVKDS MERAAARQQS IKPGPELGGE FPVQDMKTGE GGLLQVTLEG INLKFMHNQF LKLKKW
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Madd Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Andere Bezeichnung: Madd (MADD Produkte)

Synonyme: MADD Protein, MGC145371 Protein, DENN Protein, IG20 Protein, RAB3GEP Protein, 9630059K23Rik Protein, MAP kinase activating death domain Protein, MAP-kinase activating death domain Protein, MADD Protein, madd Protein, Madd Protein

Hintergrund: Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis). {ECO:0000269|PubMed:14735464, ECO:0000269|PubMed:15007167}.

Molekulargewicht: 176.0 kDa Including tag.

UniProt: Q80U28

Pathways: Caspase Kaskade in der Apoptose