MROH2B Protein (AA 1-1581) (Strep Tag)

Produktnummer ABIN3135909

Produktdetails

Target: MROH2B (Maestro Heat-Like Repeat Family Member 2B (MROH2B))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1581

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses MROH2B Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MEEYGDMFGD INLTIGMLSK EDNISKEDIY CHLTSFIQNT DIMDDAIVQR LIYYTSKDMR DEEIPRELRM LAGEVLVSLA AHDFNSVMYE VQSNFRILEL PNEFVVLALA ELATSYVSQS IPFMMMTLLT MQTMLRLVED ENMRQTFCIA LENFSKSIYK YVNHWKDFPY PKLDANRLSD KIFMLFRYIM EKWAPQASPM HALAIIKAHG PTVSLLLHRE DFCEFALSQI SWLLLQYRDK ENDFYITQSL KQILTAAVLY DIALPKNLRR SVLSSLLHQI CKVPEPPIKE NKLEASSCFL ILAHANPVDL LDFFDEQIRS TNEAVRTGIL TLLRSTINAE EPKFRNHTTS IEKTVKLVMG DLSVKVRKST LLLIQTMCEK GYIEAREGWP LIDYIFSQFA MSNRNLENPI KSNSQEDENG EKSVQETSLE VLKSLDPLVI GMPQVLWPRI LTYVVPKEYT GTLDYLFNII RILIMAEEKK KRDIQESTAL VVSTGAVKLP SPQQLLARLL VISILASLGQ LCGAGAIGLL KIMPEIIHPK LAEMWKTRMP ALLQPLEGSN ASIVLWETML LQLLKESLWK ISDVAWTSQL SRDFSLQMGS YSNSSMEKKF LWKALGTTLA SCQDKDFVSS QINEFLVTPS LLGDHRQGTT SILGFCAENH LDIVLNVLKT FQDKEKFFVN RCKGIFSGKK SLTKTDLILI YGAVALHAPK QQLLARLDQD IMGQILLLYG QCCQILGVSV INKDMDLQMS FTRSITEVGI AVQDAEDQKF QFTYKEMLIG SMLDLIKDEP LNTLASPVRW KVLIAIRYLS KLKPALSLND HLNILEENIR RLLPLPPLEK LKSQGETDKD RERIEFLYER SMDALGKLLR SMIWDNTDAQ NCEEMFNLLR MWLVSQKQWE RERAFQVTSK VLTKDVEAPQ NFRIGSLLGL LAPHSCDTLP TIRQAATSST IGLLCAKGIC QEVDRLQGLQ EGLDSEDEQV QIKISSKIAK IVCKFIPSEE IQVFLEETLD GLETLDPLCT KACGIWMIAA LKEHGALLED QLLEILSTIY HHMPVLRQKE ESFQFMLEAI SQIASFHMDA VVNNLLQKPL PFDRDTKTLW KALAENPASS GKLMRALIKK LVARLEDDIA GTEAISVACA IYEVILTGAH ITHLYPELFT LLLKLVSCSL GQKMPMSTLS QRRRVMQLGE RQRFPDPCRL STATLKCLQA QAMREGLAKE SDEGDNLWTL LSNPDTHHIG VCALARSMAV WQHGVILDIM EHLLSSLTSS SENYRITGMA FFSELMKEPI LWKHGNLRDV LIFMDQNARD SNAILRQMAI RGLGNTACGA PHKVRKYKQM MLECIIRGLY HLARTEVVCE SLKALKKILE LLTERDINFY FKEIVLQTRT FFEDEQDDVR LTAISLFEDL ATLTGRRWKI FFAEEVKKSM ISFLLHLWDP NPKIGAACRD VLVICIPFLG LQELYGLLDH LLERDLPRAR DFYRQLCMKL SKKNQEILWI LHTHSFTFFT SSWEMIRSAA VKLTDAIILH LTKRYVELLD REQLTMRLQA LRQDPCISVQ RAAEATLQTL LRRCKEISIP L
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: MROH2B (Maestro Heat-Like Repeat Family Member 2B (MROH2B))

Andere Bezeichnung: Mroh2b (MROH2B Produkte)

Synonyme: HEATR7B2 Protein, 4930455B06Rik Protein, Heatr7b2 Protein, c11.1-like Protein, RGD1305413 Protein, maestro heat like repeat family member 2B Protein, maestro heat-like repeat family member 2B Protein, MROH2B Protein, Mroh2b Protein

Hintergrund: Maestro heat-like repeat-containing protein family member 2B (HEAT repeat-containing protein 7B2) (Sperm PKA-interacting factor) (SPIF),FUNCTION: May play a role in the process of sperm capacitation (PubMed:27105888). {ECO:0000269|PubMed:27105888}.

Molekulargewicht: 180.5 kDa

UniProt: Q7M6Y6