KIAA1211 Protein (KIAA1211) (AA 1-1207) (His tag)

Produktnummer ABIN3135209

Produktdetails

Target: KIAA1211

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1207

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses KIAA1211 Protein ist gelabelt mit His tag.

Applikation: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MGARAFSHDS IFIPDGGAES EQTVQAMSQD NILGKVKTLQ RQLGKNIKFG QRPSNAIPMK KAGSTDASSE EDFVLTSPME IVTQQDIVPS DTENKSSDTP SSSSPLNLPE AGSDMEEKVA PVKPSRPKRH LSSAGTIESV NLDAIPLAIA RLDNSAARHK LAVKPKNQRV SRKHRWLAQD RQNEPGSFES QSSLDQNGQL GEDKHIWHGE EPEPLESHEE KRLHEEYWRE LEAKCKRQKA EAAEKRRQEE QRRQALERRL WEESLRQELL EEEEEGEEEE EVKEEGEEGE EVGLQPRAGK VPPEEGHQSG PEEQRCTEQE LGGADDPARL EAEERRREQE EAERQAEKLR QREAESQEEE LRQREAERQR AQEEDAKGML QEEEEEAKRI EELKGKETPE PLVEEGPQSP EGESQPWLTD DADQRSPLQR DLEKPGERER EDLESAGQRE IAEEPRGEGE PAEQSGDLDA HCGGVDGEGK ETAQTDSPQP QERQMEGTPA PEENEATAAD IDRKVEELRW QEVDERQTMP RPYTFQVSSG SRQILFPKVN LSPVTPAKDA SLAPAAQEPP APRGAASHAL PSALSIPHTA ILVTGAQLCG PAVNLSQIKD TACKSLLGLS EEKRPMDVPT VESRAGSGKS RPAPESPSNA AALAEWASIR SRILKNSEGD QRGDREPARA GDEPVPRARC DSRGNVRRTP PVNAKFSIMP AWQKFSDSGA ETFRQSLDGE SGRKKPGLAP SEETAPQPHA AAQQEVSQEP PDTTDGCKFA KDLPSFLVPG LPSPQKAASR TESTTTLDSE TTSDVGNPDP AMPGGEEKAS PFGIKLRRTN YSLRFHCDQQ AEQKKKKRHS STGDSVGGAT PATGSVSGES EPEATFLKHG PSLPQERKPA LSPRKDSAES HSSGHYVAVA QSGLPPASGQ TPAPEQDRAV SKMPSMQKPA LAPKPASQTP PSSPLSKLSR PHLVELLARR AGKLDSEPSE TAKESSDNQP PSPSLPEELK GQKRDEKDVP EKKPASPPLP AGQQERPSLI PETGRKEKPV LQSRHSLDGS KVTEKVETAQ PLWITLALQK QKGFREQQAT REERKQAREA KQAEKLSKET VSVSLQPGSS RASKTAPVHK PAAPSEEKKP ETAVSRLQRR EQLKKSNTLP TSVTVEISDS APSAALVKDV TKRFSTPDAA PVSTEPAWLA LAKRKAKAWS DCPQIIK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Kiaa1211 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: KIAA1211

Andere Bezeichnung: Kiaa1211 (KIAA1211 Produkte)

Synonyme: 2810489J07Rik Protein, 4930529A19Rik Protein, 4931405B09Rik Protein, Kiaa1211 Protein, mKIAA1211 Protein, KIAA1211 Protein, RIKEN cDNA C530008M17 gene Protein, KIAA1211 Protein, C530008M17Rik Protein

Molekulargewicht: 133.2 kDa Including tag.

UniProt: Q5PR69