NLRC3 Protein (AA 1-1064) (Strep Tag)

Produktnummer ABIN3152054

Produktdetails

Target: NLRC3 (NLR Family, CARD Domain Containing 3 (NLRC3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1064

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses NLRC3 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MRRRYSHDPP GSFRETKVFG FRGEYGCKAL VDLLAGKGSQ LLQVRDKMPD SPLGSQSNES RIPKHSEALL SRVGNDPELG SPSHRLASLM LVEGLTDLQL KEHDFTQVEA TRGVWHPARV ITLDRLFLPL SRVSIPPRVS LTIGVAGVGK TTLVRHFVHC WARGQVGKGF SRVLPLTFRD LNTYEKLSAD RLIQSIFSSI GEASLVATAP DRVLLVLDGL DECKTPLEFS NTMACSDPKK EIQVDHLITN IIRGNLFPEI SVWITSRPSA AGQIPGGLVD RMTEIRGLTE EEIKVCLEQM FPEEQNLLGQ VLSQVQANRA LYLMCTVPAF CRLTGLALGH LYRTRLAVQD IELPLPQTLC ELYSWYFRMA LGGEGQDKEK VSPRIKQVTQ GARKMVGTLG RLAFHGLVKK KYVFYEQDMK AFGVDLALLQ NTLCSCLLQR EETLASSVAY CFIHLSLQEF VAATYYYSAS KRAIFDLFTE SGMSWPRLGF LAHFRCAAQR ATQAKDGRLD VFLRFLSGLL SPRVNTLLAG SLLSQGEHQS YRDQVAEVLQ GFLHPDAAVC ARAINVLYCL SELRHTELAC SVEEAMRSGT LAGMTSPSHR TALAYLLQMS DICSPEADFS LCLSQHVLQS LLPQLLYCQS LRLDNNQFQD PVMELLGSVL SGKDCRIRKI SLAENQIGNK GAKALARSLL VNRSLITLDL RSNSIGPPGA KALADALKIN RTLTSLSLQS NVIKDDGVMC VAEALVSNQT ISMLQLQKNL IGLIGAQQMA DALKQNRSLK ALMFSSNTIG DRGAIALAEA LKVNQILENL DLQSNSISDM GVTVLMRALC SNQTLSSLNL RENSISPEGA QALTQALCRN NTLKHLDLTA NLLHDRGAQA IAVAVGENHS LTHLHLQWNF IQAGAARALG QALQLNRTLT TLDLQENAIG DEGASSVAGA LKVNTTLIAL YLQVASIGSQ GAQALGEALT VNRTLEILDL RGNDVGAAGA KALANALKLN SSLRRLNLQE NSLGMDGAIF VASALSENHG LHHINLQGNP IGESAARMIS EAIKTNAPTC TVEI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: NLRC3 (NLR Family, CARD Domain Containing 3 (NLRC3))

Andere Bezeichnung: Nlrc3 (NLRC3 Produkte)

Synonyme: NLRC3 Protein, nod3 Protein, NLR-A3 Protein, NOD3 Protein, CLR16.2 Protein, D230007K08Rik Protein, mFLJ00348 Protein, Nlrc3-ps1 Protein, RGD1304902 Protein, NLR family CARD domain containing 3 Protein, NLR family, CARD domain containing 3 Protein, NLRC3 Protein, nlrc3 Protein, Nlrc3 Protein

Hintergrund: Protein NLRC3,FUNCTION: Negative regulator of the innate immune response. Attenuates signaling pathways activated by Toll-like receptors (TLRs) and the DNA sensor STING/TMEM173 in response to pathogen-associated molecular patterns, such as intracellular poly(dA:dT), but not poly(I:C), or in response to DNA virus infection, including that of Herpes simplex virus 1 (HSV1) (PubMed:22863753, PubMed:24560620). May affect TLR4 signaling by acting at the level of TRAF6 ubiquitination, decreasing the activating 'Lys-63'-linked ubiquitination and leaving unchanged the degradative 'Lys-48'-linked ubiquitination (PubMed:22863753). Inhibits the PI3K-AKT-mTOR pathway possibly by directly interacting with the posphatidylinositol 3-kinase regulatory subunit p85 (PIK3R1/PIK3R2) and disrupting the association between PIK3R1/PIK3R2 and the catalytic subunit p110 (PIK3CA/PIK3CB/PIK3CD) and reducing PIK3R1/PIK3R2 activation. Via its regulation of the PI3K-AKT-mTOR pathway, controls cell proliferation, predominantly in intestinal epithelial cells (PubMed:27951586). May also affect NOD1- or NOD2-mediated NF-kappa-B activation (By similarity). Might also affect the inflammatory response by preventing NLRP3 inflammasome formation, CASP1 cleavage and IL1B maturation (By similarity). {ECO:0000250|UniProtKB:Q7RTR2, ECO:0000269|PubMed:22863753, ECO:0000269|PubMed:24560620, ECO:0000269|PubMed:27951586}.

Molekulargewicht: 116.0 kDa

UniProt: Q5DU56