NEXMIF/KIAA2022 Protein (AA 1-1515) (His tag)

Produktnummer ABIN3135179

Produktdetails

Target: NEXMIF/KIAA2022 (NEXMIFKIAA2022) (Neurite Extension and Migration Factor (NEXMIFKIAA2022))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1515

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses NEXMIF/KIAA2022 Protein ist gelabelt mit His tag.

Applikation: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MDNQQDKVIA ASANGDNNLI NGVKNNDSED QEVAMKSFVA LEATTPIQPI PVIQKESPMF PRGLLPPPSK KPCMQSPPSP LALIEAPDHS ANSASVNAIS LTSGVAKGLN TWSLPNECEK APFAIMEPAG MSALNGDCLM QPSRTCLGCF MESKEAVDPE PGISLKVSDL NRDYETCAVS DIGIQCINAG ENIKYGEQLL SDQLLGFPLH KSRAGDRRES EKPDIDLEDP TQKSYYEALL LDKCNTEEAL LANSNQDWGY FETFISESKI ELLDLCSKNE LSVNLFSEED VENYMFDDDE STLGSDVCSL KIRYESFQDN VRDKTTLLMQ EDAQFNFFPS VFTTCPKRES KSGILKQSSD LSQFKVPDVS IIWGEEDKNL DKKKGKEEVH EDKSIETKDE KDNGEKPALN NKPCGGLEVE QFKNLKADQL TNSLETSGNF SDDSSFIEVS YDAMGEIKDC SRYMARDTNS GSSSSQQNYG LRAKRKVRYS EDYLYDVDSL EGEKVNERKE WPPGGSKEED DDEWCPKKRR KVTRKEPPVI IKYIIINRFK GEKNMLVKLS KVDASETTVN LSENQLSKYA KLSPLKGFWQ KKKKQKNSNT DSVKTPLCQK QSFEPGSFEV SFLPPARKRK SKLGNRHRIQ RIQSVETSAS SKQVSFCSDQ KQACNRKEDG VKGTPKSALL TDPSCANGSH LRGLIVSDSV KVKAQDTEFK GPERKVLNKI KFKSEARLKS KKIKAGQENK PVVQMSPVSE DTSSKANLKN EVTPGTSNSS HMSEFHETKV KNSTFLPTTC SSEMPLSSAN VATNIPVIPG GYLQTLLDAS DLSNNTSISY FTNHSAEQNE GSLTQTEKAF VPLQSAQDCV LSSSSDSQLQ QSSQNFKMEA SNFGSLWPDK DTSGSQEFMT EVSREIATNQ SSEFEASQVV SMENNLTAIT YSPVCLNSDA SGCNKVLYAS LQDSHLPPED LYQLCHFNNG EICFPFQQGP LSTDDDGRLF SFDSMTSLTV SSSNYCSLSL KSCEKDGDDE INDDFLAHCS PKLVIQQSID EIAPLKESTD LLDISNFTPD KFRHSSLLEM SPPDTPSLSP QSTRCESIKT LGTMKGFQEG VPGSLSTVEK IKWDCNTLSQ QAQADDGFTL NSHQFQFHMF NDEDSVGLLQ KSPCLSTFDE PAGQINTNSK VSKSRKKTSP GKSGAVSQSS SQKNSRKKSP KASNKGVEKP PSKTSRQVPK STKKGKYVAA VNGEKMQIGI GHSGGQPNST SSNAKTLTEC IQHGGPVASM KIPSQKGLSG DWALGKESRP GWNDMSVVTN TNNLLDDDQR EFQEPSYILS NIASGMADVQ RFMMASMEPL WEPMEHQGES NTFYSPDSNS LKLKTLKILA GTPQESKKKV TNGSSGATKN HRSVKAVSKS NGKAAIGEPG HADMPGSSED SRSAFFDKKY SNVNTLGNNG PTHKKLYRHK SSSKGLRDEK YKGKRVEREQ AHKDEAGTTS FEKLRDSNYN LLKAETAFGV LPVFEEETHI FQKDI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Kiaa2022 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: NEXMIF/KIAA2022 (NEXMIFKIAA2022) (Neurite Extension and Migration Factor (NEXMIFKIAA2022))

Andere Bezeichnung: Kiaa2022 (NEXMIFKIAA2022 Produkte)

Synonyme: A230051P11 Protein, C77386 Protein, Kiaa2022 Protein, Xpn Protein, mKIAA2022 Protein, neurite extension and migration factor Protein, Nexmif Protein, NEXMIF Protein

Hintergrund: May be involved in neuronal development. {ECO:0000250}.

Molekulargewicht: 168.1 kDa Including tag.

UniProt: Q5DTT1