FHDC1 Protein (AA 1-1149) (Strep Tag)

Produktnummer ABIN3135067

Produktdetails

Target: FHDC1 (FH2 Domain Containing 1 (FHDC1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1149

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses FHDC1 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MHVMNCVSLA SDKENGTLAT AAAFMTGQTS PSASPPPPPP PPPPPPCPHS GEGFSPSPPP PLPPPLPGGP PIPPPPPPGL PSVSYLNGYS SLGKKKRMRS FFWKTIPEEQ VRGKTNIWTL AAKQQHQYQI DKKTIEELFG QQEDTSKASL PKRGGALNSS FRDAREEVTV LDAKRSMNIG IFLKQFKKSP QSIVEDIYQG KSEHYGSETL REILKLLPES EEVKKLKAFN GDVSKLSLAD SFLHCLIQVP NYSLRIEAMV LKKEFLPSCS SLFKDIRTLR AATKELMLCE ELHSILHLVL QAGNIMNAGG YAGNAVGFKL SSLLKLADTK ANKPGMNLLH FVAQEAQKQD AILLNFSEKL QHVQETSRLS LDITEAELHS LFVRTKSLQE NIQLDQELCQ QMEDFLQFAV EKLAELELWK RELQGEAHTL IDFFCEDKET MKLDECFQIF RDFCTRFNKA VKDNHDREEQ ERKQLQRQKE MEQKRYSWST GELGSFGRSS SENDVQMLAK TGTEDLPSFL KPRPNSPSYR PPNTRRSRLS LGISADRELL TFLESATSSP EDPNKFNSLP RSSPRQARPT IAWMEPREQQ SHGPNFTHEP QASKIQEKAP PPAWQNQLPT TWREEPASPL PLAGRSRPSL RKRNSEPVGL GPTQSPPLLP LDLGVREHEL VTGLTQFDLQ SPKSLEEGSQ LTLNDFCPTK LPSPGDRSSQ PFAAGGDSLP PKDTDTQEVL SPAGEDDRTI SDEPSSEALV SVVVTDTEDK DAGPLLYVSD TTDCSLTLDC SEGMDSRAGG DKQEEEKEGD GSVSSGAGEA GSSQVSSNSV SSPPGEVPAP KSSKSELSCQ GGLPKDRPSR GKDAIAPKRN SFKEASVGAS KPVSARRSQG VTTKPVRTLN SSENEHMRKV VPISKSSRGA GPWKRPEPTP KATPRETPSS TDTPLSRRSS VRGTSDTSPR RPQVSGSGAE EPRLPRSSGS ISGRPGKDAP LQPRASFRKP SAKPLRNIPR QKPEENKVSS PNSPDPESPK EEPKAPQATG VSRALPPIPS FARNTVASSS RSLRTDAPPA ARTTGLTRTV SQRQLRVKGG SEDSASKDIG TLKRASSARA SKKCPESAGG SSANVETSLK GRGTTERSSL RLKDSGQATL GRILRPLQK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: FHDC1 (FH2 Domain Containing 1 (FHDC1))

Andere Bezeichnung: Fhdc1 (FHDC1 Produkte)

Synonyme: RGD1311955 Protein, 6330505N24Rik Protein, Gm126 Protein, FH2 domain containing 1 Protein, Fhdc1 Protein, FHDC1 Protein, fhdc1 Protein

Hintergrund: FH2 domain-containing protein 1 (Inverted formin-1),FUNCTION: Microtubule-associated formin which regulates both actin and microtubule dynamics. Induces microtubule acetylation and stabilization and actin stress fiber formation (PubMed:18815276). Regulates Golgi ribbon formation (PubMed:26564798). Required for normal cilia assembly. Early in cilia assembly, may assist in the maturation and positioning of the centrosome/basal body, and once cilia assembly has initiated, may also promote cilia elongation by inhibiting disassembly (PubMed:29742020). {ECO:0000269|PubMed:18815276, ECO:0000269|PubMed:26564798, ECO:0000269|PubMed:29742020}.

Molekulargewicht: 125.4 kDa

UniProt: Q3ULZ2