Cingulin Protein (CGN) (AA 1-1191) (Strep Tag)

Produktnummer ABIN3134554

Produktdetails

Target: Cingulin (CGN)

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1191

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses Cingulin Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MADPRGPVDH GVQIRFITEP EGATEMGTLR RSGRRPARDA RASTYGVAVR VQGIAGQPFV VLNSGEKGTD SFGVQIKGGN NRGSPGALSS DSELPENPYS QVKGRPATSR SSTSDEEPKD HLNGKLIRSQ SQASLTGLAF MSPSNRSTSL LELAPKPTSS INTIDTAPLS SVDSLINKFD SQKGGQVRGR TGRRTRTLPH EQRKRSQSLD SRLPRDTREE REHQSANHWT RGTKYDNHVD SSKNPSQKQS PFSSFSRSRQ TQDWVLQSFE ETRDPAMVQF KSTPDLLRDQ RETAPPGSAD HVKATIYGIL REGSSESEAS VRRKVSLVLE QMQPLGMVSP ASTKALAGQA ELTRKMEELQ KKLDEEVKKR QKLEPSRVGL ERQLEEKAEE CHRLQELLER RKGEVQQSSK ELQNMKLLLG QEEGLRHGLE AQVKELQLKL KHSQSPDSGK ESLLKDLLDT RELLEELLEG KQRVEEQLRL RERELTALKG ALKEEVASHD QEVEHVRLQY QRDTEQLRRS MQDATQDHAA LEAERQKMSS LVRELQRELE ETSEETGHWQ SMFQKNKEEL RATKQELLQL RMEKEEMEEE LGEKMEVLQR DLEQARASTR DTHQVEELKK ELRRTQGELK ELQAEQQNQE VTGRHRNQVL EKQLAALREE ADRGRELEQQ NLQLQKTLQQ LRQDCEEASK AKVASETEAM MLGQRRATVE TTLRETQEEN DEFRRRILGL EQQLKEARGL AEGGEAVEAR LRDKVHRLEV EKQQLEEALN AAQEEEGNLA AAKRALEVRL DEAQRGLARL GQEQQALNRA LEEEGKQREA LRRSKAELEE QKRLLNRTVD RLNKELEQIG DDSKLALQQL QAQMEDYKEK ARKEVADAQR QAKDWASEAE KNSGGLSRLQ DELQRLRQAL QTSQAERDTA RLDKELLAQR LQGLEQEAEN KKRFQDDKAR QLKSLEEKVS RLEAELDEEK NTVELLTDRV NRGRDQVDQL RTELMQERSA RQDLECDKIS LERQNKDLKT RLASSEGFQK PSASLSQLES QNQLLQERLQ AEEREKTVLQ STNRKLERRV KELSIQIDDE RQHVNDQKDQ LTLRVKALKR QVDEAEEEIE RLDSLRKKAQ RELEEQHEVN EQLQARIKSL EKDAWRKASR SAAESALKQE GLSSDEEFDN VYDPSSIASL LTESNLQTSS C
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Cingulin (CGN)

Andere Bezeichnung: Cgn (CGN Produkte)

Synonyme: CGN Protein, cgn Protein, 6330408J11Rik Protein, AI647528 Protein, AI987749 Protein, cb117 Protein, im:7137721 Protein, sb:cb117 Protein, sb:cb117. Protein, si:dkey-204a24.2 Protein, cingulin Protein, cingulin L homeolog Protein, cingulin b Protein, CGN Protein, Cgn Protein, EDI_031160 Protein, cgn Protein, cgn.L Protein, cgnb Protein

Hintergrund: Cingulin,FUNCTION: Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. {ECO:0000250}.

Molekulargewicht: 136.4 kDa

UniProt: P59242