TKT Protein (AA 1-623) (Strep Tag)

Produktnummer ABIN3133959

Produktdetails

Target: TKT (Transketolase (TKT))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-623

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses TKT Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequenz: MEGYHKPDQQ KLQALKDTAN RLRISSIQAT TAAGSGHPTS CCSAAEIMAV LFFHTMRYKA LDPRNPHNDR FVLSKGHAAP ILYAVWAEAG FLPEAELLNL RKISSDLDGH PVPKQAFTDV ATGSLGQGLG AACGMAYTGK YFDKASYRVY CMLGDGEVSE GSVWEAMAFA GIYKLDNLVA IFDINRLGQS DPAPLQHQVD IYQKRCEAFG WHTIIVDGHS VEELCKAFGQ AKHQPTAIIA KTFKGRGITG IEDKEAWHGK PLPKNMAEQI IQEIYSQVQS KKKILATPPQ EDAPSVDIAN IRMPTPPSYK VGDKIATRKA YGLALAKLGH ASDRIIALDG DTKNSTFSEL FKKEHPDRFI ECYIAEQNMV SIAVGCATRD RTVPFCSTFA AFFTRAFDQI RMAAISESNI NLCGSHCGVS IGEDGPSQMA LEDLAMFRSV PMSTVFYPSD GVATEKAVEL AANTKGICFI RTSRPENAII YSNNEDFQVG QAKVVLKSKD DQVTVIGAGV TLHEALAAAE SLKKDKISIR VLDPFTIKPL DRKLILDSAR ATKGRILTVE DHYYEGGIGE AVSAAVVGEP GVTVTRLAVS QVPRSGKPAE LLKMFGIDKD AIVQAVKGLV TKG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TKT (Transketolase (TKT))

Andere Bezeichnung: Tkt (TKT Produkte)

Synonyme: TK Protein, TKT1 Protein, p68 Protein, Dmel\\CG8036 Protein, anon-WO0118547.344 Protein, cb860 Protein, fb38f03 Protein, fj52f12 Protein, id:ibd3270 Protein, wu:cegs2794 Protein, wu:fb38f03 Protein, wu:fj52f12 Protein, tkt1 Protein, PSPTO0385 Protein, Tb08.11J15.550 Protein, xcc-b100_0966 Protein, transketolase Protein, CG8036 gene product from transcript CG8036-RB Protein, TransKeTolase homolog Protein, transketolase b Protein, transketolase L homeolog Protein, transketolase Tkt Protein, tkt Protein, TKT Protein, Tkt Protein, CG8036 Protein, tkt-1 Protein, tktb Protein, tkt.L Protein, tkt Protein, JK_RS05135 Protein, Tb927.8.6170 Protein, APH_RS01480 Protein

Hintergrund: Transketolase (TK) (EC 2.2.1.1) (P68),FUNCTION: Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate. {ECO:0000250}.

Molekulargewicht: 67.6 kDa

UniProt: P40142