CD101 Protein (CD101) (AA 21-970) (His tag)

Produktnummer ABIN3130959

Produktdetails

Target: CD101

Protein-Typ: Recombinant

Proteineigenschaft: AA 21-970

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses CD101 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: QREVKIQEGP LYRAEGYPVS IRCTVSGHQG PSTQDFRWSI YLPSAPTKEV QIISTKDAGF SYAVYAQRVQ SKEIYIERLQ GDSVLLHISK LQMKDAGEYE CHTPNTDGKY FGSYSAKTNL TVVPDTLSAT MPSQTLSKKE GEPLELTCET TKATVQHTHL SLTWYLMQEG GGSQATEIVS LSKDFVLTPG SSYADRFVAG DVRLDKLGAT SFRLSVGKLQ PSDQGQVFCE ATEWIQDPDE TWTLITRKQT DQTALRIQPA ARDFTVSITA SSSPDEGKPL ELVCLAVGRD GNPQLQGVWF LNGKEIAQTD AGGVLDLKRD YRDRASQGQL QVSKLSAQTF SLKIFSVGPE DVGTYSCEVA EVARTQMGSW QVLQRKQSPG YRVQLREPAA RSVTVSAEQR TVWEGETLTL LCKAAGDVSA LSVSWWLTPQ DQSTPVFVAG MGQDGTVQLG VSSPGPAHRG NRRLEKVDWA TFRLEIASAM VTDSGTYECR VSERLQNQAK GLQSTQKISV TVKSLKSSLR VNLMSRQPQV MLAHTFHLSC VVRANYSDLK LPFSVTWQFQ PAGSGAFHRL IRIAHNGTVE WGDVLSQIHR KTKVSQSFFR SQLQIYDAAM EETGVYRCTV EVYDRDSICT SGPARVSATS NLLMITVTFP ESKLSVNSSS QVQELSISSS TQIECAILSR SAGNLPLSII WYFSSVSANA SYLKILEMDQ SSVVKYGDEF QTPRSKQKFY SEKVSQDLFL LNILSVEDSD QGHYHCAVEE WLLSTNDTWQ KLERKTSGLT ELKLRPTGSQ VHVSKVNWTG NATEYGEAGF SCSLDGSGST ASLYSVTWYR GRGTATATAA AVANATATIT APAGSQMLVH LQYDGLLQYG REGSRRLQHC YRSSPTDFVL KLHRVEMEDA GIYWCRVTEW QQHGHPGKWI NQASGESQRM VLRVLRSEPT VSSLICSSGP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Cd101 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CD101

Andere Bezeichnung: Cd101 (CD101 Produkte)

Synonyme: Gm1016 Protein, Gm734 Protein, Igsf2 Protein, EWI-101 Protein, IGSF2 Protein, V7 Protein, CD101 molecule Protein, CD101 antigen Protein, CD101 Protein, Cd101 Protein

Hintergrund: Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells (By similarity). {ECO:0000250}.

Molekulargewicht: 105.9 kDa Including tag.

UniProt: A8E0Y8