MYOM3 Protein (AA 1-1439) (Strep Tag)

Produktnummer ABIN3130717

Produktdetails

Target: MYOM3 (Myomesin 3 (MYOM3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1439

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses MYOM3 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MTLPHSPGSA GEPQASQTVQ VHRLEHRQEE EQKEERQHSL QMGSSVQRRT YRSSEEEQQF SSEDYALAAA LALTASSELS WEAKLRRQTT TVELEERGQR RVGFGNDLER MELAFLRTQR LLRQRRDWKA LRQRTEEKVR EAKELIELCS GRGPWFWIPL RSHAVWEHTT VLLTCTVQGS PPFQVTWYKN DIRIDPRLFP AGKYRITNNY GLLTLEIMRC TVEDSATYTV LVKNAYGQAS SFAKVLIRNY LGKDAGFDSE IFKRSMFGPS AEFTSVLKPI FAQEKEPFSL TCLFSDDVLE AEQRIQWYRD GRLLRSSTRR QILYADRQAS VKVSCAYKED EGFYTIRVSS PFGPQEQSAY VFIRDAAAEK PGAPGSPLNV RCLNVHRDCL TLTWVPPSDT RGSTITGYSI EMCQGDSEEW MPCLKAPGGT CRCPIQGLVE GQSYQFRVRA ISKAGTSLPS KASEAVVTGD YDAVHKSTEI PYDLGSKITI SKNDFEDAVT IPSAPTNVHA SEIREAYAVL SWEEPRPRGR APLTYTLEKS VIGSGTWEAI STETPIKSPR FALLDLEKGK SYVFRVRALN QYGMSDPSEP SEPVALKGKP ATLPPPAQVQ AFRNTQTSVS LAWEPVDGGS ELLGYYIYSR EAGASEWQTV NNKPIQDTKF TVPGLRTGKE YDFCIRSVSE AGVGESSAAT QPVRVKQALA TPSAPYDFAL LNCGKNEMVI GWKPPKRRGG GKILGYFMDQ HDSVESDWHP VNRQPIPSRV CKVTNLHEGH FYEFRARAVN WAGIGELSAP SSLFECKEWT MPEPGPPYDV RVSEVQATSV MLQWEPPLYI GAGPVTGYHV SFQEKGSEEW KPVTPDATSD THLRVSDLQP GKQYMFRVQA MNSAGLGQPS VPTDPVLLED KPDAQEIEVG VDDEGQIYLA FEAPEAPDFP EFQWSKDYQG PPDPQRVEVE DEISKSKVIL KEPDLQDLGI YSVVVPDADE DTSASHTLTE EELNKLKKLS HEIRNPVIKL ISGWNVEILE QGEVRLWLEV EKLSPAAELH LIFNEKEIFS SPNRKINFDR EKGLVEVIIQ QLSEDDKGSY TAQLQDGKAK NQITLALVDD EFDKLLRKAD AKRRDWKRKQ GPYFQEPLTW KVTDDCQVLL SCKVTNTKKE SRFQWFFQKK EAPHGQYNPP TGDGSLSIEG FSKENQGVYR AVVSDERGED DTVLDLTGEA LDAVLTELGR IGALSATPLK IQGTEEGIRL FSKVKYYNVD YMKTAWFHKD KRLESGDRVR AGTTLDEIWL HILDPKDSDK GKYTLEITAG KEVRQLSADL SGQAFDDALA EHQRLKALAV IEKNRAKVVR GLPDVATIME DKTLCLTCVI SGDPSPEISW LKNDQPISFF DRYHMEVKGT EVTVTIDKVT SEDSGRYGIF VKNKYGSETG QVTISVFKHG EEPKELKKK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: MYOM3 (Myomesin 3 (MYOM3))

Andere Bezeichnung: Myom3 (MYOM3 Produkte)

Synonyme: RP11-293P20.1 Protein, 8430427K15Rik Protein, Gm434 Protein, myomesin 3 Protein, myomesin family, member 3 Protein, Myom3 Protein, MYOM3 Protein

Hintergrund: Myomesin-3 (Myomesin family member 3),FUNCTION: May link the intermediate filament cytoskeleton to the M-disk of the myofibrils in striated muscle. {ECO:0000250}.

Molekulargewicht: 161.8 kDa

UniProt: A2ABU4