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UQCRC2 Protein (AA 15-453) (His tag)

Crystallography grade UQCRC2 Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Produktnummer ABIN3129531
  • Target Alle UQCRC2 Proteine anzeigen
    UQCRC2 (Ubiquinol-Cytochrome C Reductase Core Protein II (UQCRC2))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 15-453
    Spezies
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Quelle
    • 6
    • 3
    • 2
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses UQCRC2 Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequenz
    SLKVAPKVKT SAAPGGVPLQ PQDLEFTKLP NGLVIASLEN YAPLSRIGLF VKAGSRYEDS NNLGTSHLLR LASSLTTKGA SSFKITRGIE AVGGKLSVTA TRENMAYTVE GIRSDIEILM EFLLNVTTAP EFRRWEVAAL RSQLKIDKAV AFQNSQTRII ENLHDVAYKN ALANPLYCPD YRMGKITSEE LHYFVQNHFT SARMALVGLG VSHSVLKQVA EQFLNMRGGL GLAGAKAKYR GGEIREQNGD NLVHAAIVAE SAAIGNAEAN AFSVLQHLLG AGPHIKRGNN TTSLLSQSVA KGSHQPFDVS AFNASYSDSG LFGIYTISQA AAAGEVINAA YNQVKAVAQG NLSSADVQAA KNKLKAGYLM SVETSEGFLS EIGSQALAAG SYMPPSTVLQ QIDSVADADV VKAAKKFVSG KKSMAASGNL GHTPFLDEL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Uqcrc2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product UQCRC2 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    UQCRC2 (Ubiquinol-Cytochrome C Reductase Core Protein II (UQCRC2))
    Andere Bezeichnung
    Uqcrc2 (UQCRC2 Produkte)
    Synonyme
    MC3DN5 Protein, QCR2 Protein, UQCR2 Protein, fi06a11 Protein, uqcrc2 Protein, wu:fb64g04 Protein, wu:fi06a11 Protein, fb33d01 Protein, fc43c05 Protein, wu:fb33d01 Protein, wu:fc43c05 Protein, zgc:92453 Protein, MGC80228 Protein, MGC130698 Protein, Uqcrc2 Protein, Afu5g04210 Protein, AO090102000625 Protein, 1500004O06Rik Protein, UQCRC2 Protein, ubiquinol-cytochrome c reductase core protein 2 Protein, ubiquinol cytochrome c reductase core protein 2 Protein, ubiquinol-cytochrome c reductase core protein 2b Protein, ubiquinol-cytochrome c reductase core protein II Protein, ubiquinol-cytochrome c reductase core protein 2a Protein, ubiquinol-cytochrome c reductase core protein 2 L homeolog Protein, cytochrome b-c1 complex subunit 2, mitochondrial Protein, ubiquinol-cytochrome C reductase complex core protein 2 Protein, ubiquinol--cytochrome-c reductase subunit 2 Protein, UQCRC2 Protein, Uqcrc2 Protein, uqcrc2b Protein, uqcrc2a Protein, uqcrc2.L Protein, uqcrc2 Protein, LOC100026672 Protein, LOC100389097 Protein, LOC100456655 Protein, LOC100592631 Protein, AFUA_5G04210 Protein, ANI_1_872084 Protein, AOR_1_1048134 Protein, QCR2 Protein, LOC100861263 Protein
    Hintergrund
    This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex (By similarity). {ECO:0000250}.
    Molekulargewicht
    47.5 kDa Including tag.
    UniProt
    Q9DB77
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