CEP57L1 Protein (AA 1-400) (Strep Tag)

Produktnummer ABIN3127017

Produktdetails

Target: CEP57L1 (Centrosomal Protein 57kDa-Like 1 (CEP57L1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-400

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses CEP57L1 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MDSELSQSMV GSYLNPPERM HLPSFTQNEA FQNCHPGTPP KMFNSPNNQA LVSALKTLQE KIRRLELERT QAEDNLNLLS REAAQYKKAL EEETNERNLA HQELIKQKKD ISIQLSSAQS RCILLEKQLE YTKRMVLNVE REKTMILEQQ AQLQREKEQD QMKLHAKLEK LHVLEKECLR LTATRQTAED KIKCLEEKLK EEEHQRRLFQ DRACEKTNCL KREPPQQRDH KFRTPTFERK KPFRTTSQAR ANPQSSGEPV SICDSLSELL MTMEEELDQM NMEHRELLRQ TMQPGNHSVS EDIEQELEQL AKKMESKGDQ ISKLKKHQDS VRKLQEKIEN SRINESSGIH GNPKGSKNLK TSPRKCVSET SSFQRDRGFQ PVQVHSLQSK LRRDDIKWEQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CEP57L1 (Centrosomal Protein 57kDa-Like 1 (CEP57L1))

Andere Bezeichnung: Cep57l1 (CEP57L1 Produkte)

Synonyme: 2410017P07Rik Protein, 4930484D11Rik Protein, AI607327 Protein, cep57R Protein, RGD1311358 Protein, C6orf182 Protein, bA487F23.2 Protein, XCep57 Protein, c6orf182 Protein, cep57 Protein, cep57r Protein, chromosome 6 open reading frame 182 Protein, centrosomal protein 57-like 1 Protein, centrosomal protein 57 like 1 Protein, centrosomal protein 57kDa-like 1 L homeolog Protein, C6orf182 Protein, Cep57l1 Protein, CEP57L1 Protein, cep57l1.L Protein

Hintergrund: Centrosomal protein CEP57L1 (Centrosomal protein 57 kDa-like protein 1) (Centrosomal protein of 57 kDa-related protein) (Cep57R) (Cep57-related protein),FUNCTION: Centrosomal protein which may be required for microtubule attachment to centrosomes. {ECO:0000250}.

Molekulargewicht: 46.8 kDa

UniProt: Q8VDS7

Pathways: Maintenance of Protein Location