Glucose-6-Phosphate Dehydrogenase Protein (G6PD) (AA 17-789) (His tag)

Produktnummer ABIN3125979

Produktdetails

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Protein-Typ: Recombinant

Proteineigenschaft: AA 17-789

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses Glucose-6-Phosphate Dehydrogenase Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: QELKGHVSII LLGATGDLAK KYLWQGLFQL YLDEAGKGHS FSFHGAALTA PQQGQKLMDK VLESLSCPKD LVPSRCDELK GQFLQLSQYR QLKTVEDYQT LNKDIETQVQ QDGLWEAGRI FYFSVPPFAY ADIARNINSS CRPHPGAWLR VVFEKPFGHD HLSAQQLASE LGSFFQEEEM YRVDHYLGKQ AVAQILPFRD QNRKALDGLW NRHHVERVEI ILKETIDAEG RASFYEEYGV IRDTLQNHLT EILTLVAMEL PLNISSSAAV LQHKLWAFQA LRGLQKSSAI LGQYQAYSGQ VRRELQKPDG FQSLTPTFAG VLVHIDNLRW EGVPFILMSG KALDERVGYV RIVFKNRAYC TQSERHWVPE QSRCLPQQII FYIGHGELGH PAILVSRNLF KPSLPTQKWK EVQDQPGLRL FGRPLSDYYA YRPVREQDAY STLLSHIFHC RKESFITTEN LLASWVFWTP LLDSLAFEVP RPYPGGAENG QLLDFEFSGG QLTFSQQQLE VLIPDLGSVP KPSDFQVLGA RYRQSPLITA WPEELISKLA SDIEAAAVQA VRHFGKFHLA LSGGSSPIAL FQQLATGHYS FPWAHTHLWL VDERCVPLSD PDSNFQGLQA HLLQHVRVPY YNIHPMPVHL HQRLCAEEDQ GAQTYASEIS ALVANSSFDL VLLGMGTDGH TASLFPQSPT GLDGDQLVVL TESPFRPHQR MSLSLPLINR AKKVAVLVMG RTKREITTLV SRVGHEPKKW PISGVVPLSG QLVWYMDYEA FLG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse H6pd Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Andere Bezeichnung: H6pd (G6PD Produkte)

Synonyme: G6PD1 Protein, g6pd Protein, G6pd Protein, G6pdx Protein, g6pdh Protein, g6pd2 Protein, fj78b06 Protein, wu:fj78b06 Protein, si:dkey-90a13.8 Protein, BA3433 Protein, CORTRD1 Protein, G6PDH Protein, GDH Protein, G28A Protein, G6PD Protein, Gpdx Protein, glucose-6-phosphate dehydrogenase Protein, glucose-6-phosphate 1-dehydrogenase Protein, glucose-6-phosphate 1-dehydrogenase (G6PD) Protein, glucose-6-P dehydrogenase Protein, glucose-6-phosphate dehydrogenase L homeolog Protein, glucose-6-phosphate 1-dehydrogenase Zwf Protein, hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase Protein, glucose-6-phosphate dehydrogenase X-linked Protein, G6PD Protein, g6pd Protein, g6pD Protein, zwf Protein, LOC100120232 Protein, LACBIDRAFT_188936 Protein, G6pd Protein, g6pd.L Protein, CNG03280 Protein, Tb10.70.5200 Protein, H6PD Protein, G6pdx Protein, H6pd Protein

Hintergrund: Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates. {ECO:0000250}.

Molekulargewicht: 88.3 kDa Including tag.

UniProt: Q8CFX1

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones