NCEH1 Protein (AA 26-408) (His tag)

Produktnummer ABIN3124989

Produktdetails

Target: NCEH1 (Neutral Cholesterol Ester Hydrolase 1 (NCEH1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 26-408

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses NCEH1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)

Sequenz: AVSDPWKLML LDATFRGAQQ VSNLIHSLGL NHHLIALNFI ITSFGKQSAR SSPKVKVTDT DFDGVEVRVF EGSPKPEEPL RRSVIYIHGG GWALASAKIS YYDQLCTTMA EELNAVIVSI EYRLVPQVYF PEQIHDVIRA TKYFLQPEVL DKYKVDPGRV GISGDSAGGN LAAALGQQFT YVASLKNKLK LQALVYPVLQ ALDFNTPSYQ QSMNTPILPR HVMVRYWLDY FKGNYDFVEA MIVNNHTSLD VERAAALRAR LDWTSLLPSS IKKNYKPIMQ TTGNARIVQE IPQLLDAAAS PLIAEQEVLE ALPKTYILTC EHDVLRDDGI MYAKRLESAG VNVTLDHFED GFHGCMIFTS WPTNFSVGIR TRNSYIKWLD QNL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Nceh1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: NCEH1 (Neutral Cholesterol Ester Hydrolase 1 (NCEH1))

Andere Bezeichnung: Nceh1 (NCEH1 Produkte)

Synonyme: AADACL1 Protein, NCEH Protein, Aadacl1 Protein, B230106I24Rik Protein, CPO-BP Protein, Nceh Protein, RGD1311104 Protein, nceh Protein, aadacl1 Protein, MGC79479 Protein, nceh1b Protein, zgc:153038 Protein, zgc:92416 Protein, neutral cholesterol ester hydrolase 1 Protein, neutral cholesterol ester hydrolase 1 S homeolog Protein, neutral cholesterol ester hydrolase 1b, tandem duplicate 1 Protein, neutral cholesterol ester hydrolase 1a Protein, NCEH1 Protein, Nceh1 Protein, nceh1 Protein, nceh1.S Protein, nceh1b.1 Protein, nceh1a Protein

Hintergrund: Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. {ECO:0000269|PubMed:15840715, ECO:0000269|PubMed:16978018, ECO:0000269|PubMed:18164358, ECO:0000269|PubMed:18782767}.

Molekulargewicht: 44.0 kDa Including tag.

UniProt: Q8BLF1