XKR8 Protein (AA 1-355) (rho-1D4 tag)

Produktnummer ABIN3118151

Produktdetails

Target: XKR8 (XK, Kell Blood Group Complex Subunit-Related Family, Member 8 (XKR8))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-355

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses XKR8 Protein ist gelabelt mit rho-1D4 tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Sequenz: MPWSSRGALL RDLVLGVLGT AAFLLDLGTD LWAAVQYALG GRYLWAALVL ALLGLASVAL QLFSWLWLRA DPAGLHGSQP PRRCLALLHL LQLGYLYRCV QELRQGLLVW QQEEPSEFDL AYADFLALDI SMLRLFETFL ETAPQLTLVL AIMLQSGRAE YYQWVGICTS FLGISWALLD YHRALRTCLP SKPLLGLGSS VIYFLWNLLL LWPRVLAVAL FSALFPSYVA LHFLGLWLVL LLWVWLQGTD FMPDPSSEWL YRVTVATILY FSWFNVAEGR TRGRAIIHFA FLLSDSILLV ATWVTHSSWL PSGIPLQLWL PVGCGCFFLG LALRLVYYHW LHPSCCWKPD PDQVD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human XKR8 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: XKR8 (XK, Kell Blood Group Complex Subunit-Related Family, Member 8 (XKR8))

Andere Bezeichnung: XKR8 (XKR8 Produkte)

Synonyme: 4931440N07Rik Protein, AI597014 Protein, Gm1031 Protein, XRG8 Protein, RP11-460I13.3 Protein, X-linked Kx blood group related 8 Protein, XK related 8 Protein, Xkr8 Protein, XKR8 Protein

Hintergrund: Promotes phosphatidylserine exposure on apoptotic cell surface, possibly by mediating phospholipid scrambling. Phosphatidylserine is a specific marker only present at the surface of apoptotic cells and acts as a specific signal for engulfment. Has no effect on calcium-induced exposure of phosphatidylserine. Activated upon caspase cleavage, suggesting that it does not act prior the onset of apoptosis. {ECO:0000269|PubMed:23845944}.

Molekulargewicht: 41.4 kDa Including tag.

UniProt: Q9H6D3