GPR97 Protein (AA 21-549) (rho-1D4 tag)

Produktnummer ABIN3116239

Produktdetails

Target: GPR97 (G Protein-Coupled Receptor 97 (GPR97))

Protein-Typ: Recombinant

Proteineigenschaft: AA 21-549

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses GPR97 Protein ist gelabelt mit rho-1D4 tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: QEKPTEGPRN TCLGSNNMYD IFNLNDKALC FTKCRQSGSD SCNVENLQRY WLNYEAHLMK EGLTQKVNTP FLKALVQNLS TNTAEDFYFS LEPSQVPRQV MKDEDKPPDR VRLPKSLFRS LPGNRSVVRL AVTILDIGPG TLFKGPRLGL GDGSGVLNNR LVGLSVGQMH VTKLAEPLEI VFSHQRPPPN MTLTCVFWDV TKGTTGDWSS EGCSTEVRPE GTVCCCDHLT FFALLLRPTL DQSTVHILTR ISQAGCGVSM IFLAFTIILY AFLRLSRERF KSEDAPKIHV ALGGSLFLLN LAFLVNVGSG SKGSDAACWA RGAVFHYFLL CAFTWMGLEA FHLYLLAVRV FNTYFGHYFL KLSLVGWGLP ALMVIGTGSA NSYGLYTIRD RENRTSLELC WFREGTTMYA LYITVHGYFL ITFLFGMVVL ALVVWKIFTL SRATAVKERG KNRKKVLTLL GLSSLVGVTW GLAIFTPLGL STVYIFALFN SLQGVFICCW FTILYLPSQS TTVSSSTARL DQAHSASQE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ADGRG3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: GPR97 (G Protein-Coupled Receptor 97 (GPR97))

Andere Bezeichnung: ADGRG3 (GPR97 Produkte)

Synonyme: GPR97 Protein, PB99 Protein, PGR26 Protein, A030001G24Rik Protein, AW214383 Protein, Pb1 Protein, Pb99 Protein, adhesion G protein-coupled receptor G3 Protein, Adgrg3 Protein, ADGRG3 Protein

Hintergrund: Orphan receptor that regulates migration of lymphatic endothelial cells in vitro via the small GTPases RhoA and CDC42 (PubMed:24178298). Regulates B-cell development (By similarity). Seems to signal through G-alpha(q)-proteins (PubMed:22575658). {ECO:0000250|UniProtKB:Q8R0T6, ECO:0000269|PubMed:22575658, ECO:0000269|PubMed:24178298}.

Molekulargewicht: 60.0 kDa Including tag.

UniProt: Q86Y34