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BTN1A1 Protein (AA 27-526) (rho-1D4 tag)

Crystallography grade BTN1A1 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Produktnummer ABIN3114653
  • Target Alle BTN1A1 Proteine anzeigen
    BTN1A1 (Butyrophilin, Subfamily 1, Member A1 (BTN1A1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 27-526
    Spezies
    • 17
    • 5
    • 1
    Human
    Quelle
    • 8
    • 5
    • 5
    • 3
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses BTN1A1 Protein ist gelabelt mit rho-1D4 tag.
    Applikation
    Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
    Sequenz
    APFDVIGPPE PILAVVGEDA ELPCRLSPNA SAEHLELRWF RKKVSPAVLV HRDGREQEAE QMPEYRGRAT LVQDGIAKGR VALRIRGVRV SDDGEYTCFF REDGSYEEAL VHLKVAALGS DPHISMQVQE NGEICLECTS VGWYPEPQVQ WRTSKGEKFP STSESRNPDE EGLFTVAASV IIRDTSAKNV SCYIQNLLLG QEKKVEISIP ASSLPRLTPW IVAVAVILMV LGLLTIGSIF FTWRLYNERP RERRNEFSSK ERLLEELKWK KATLHAVDVT LDPDTAHPHL FLYEDSKSVR LEDSRQKLPE KTERFDSWPC VLGRETFTSG RHYWEVEVGD RTDWAIGVCR ENVMKKGFDP MTPENGFWAV ELYGNGYWAL TPLRTPLPLA GPPRRVGIFL DYESGDISFY NMNDGSDIYT FSNVTFSGPL RPFFCLWSSG KKPLTICPIA DGPERVTVIA NAQDLSKEIP LSPMGEDSAP RDADTLHSKL IPTQPSQGAP
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human BTN1A1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin-free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    BTN1A1 (Butyrophilin, Subfamily 1, Member A1 (BTN1A1))
    Andere Bezeichnung
    BTN1A1 (BTN1A1 Produkte)
    Synonyme
    BT Protein, BTN Protein, Btn Protein, BTN1A1 Protein, TVC Protein, butyrophilin subfamily 1 member A1 Protein, butyrophilin, subfamily 1, member A1 Protein, zgc:162154 Protein, BTN1A1 Protein, Btn1a1 Protein, btn1a1 Protein, zgc:162154 Protein
    Hintergrund
    May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane (By similarity). Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion (By similarity). {ECO:0000250}.
    Molekulargewicht
    57.3 kDa Including tag.
    UniProt
    Q13410
    Pathways
    Activated T Cell Proliferation
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