KCNJ2 Protein (AA 2-427) (rho-1D4 tag)

Produktnummer ABIN3093317

Produktdetails

Target: KCNJ2 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 2 (KCNJ2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-427

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses KCNJ2 Protein ist gelabelt mit rho-1D4 tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: GSVRTNRYSI VSSEEDGMKL ATMAVANGFG NGKSKVHTRQ QCRSRFVKKD GHCNVQFINV GEKGQRYLAD IFTTCVDIRW RWMLVIFCLA FVLSWLFFGC VFWLIALLHG DLDASKEGKA CVSEVNSFTA AFLFSIETQT TIGYGFRCVT DECPIAVFMV VFQSIVGCII DAFIIGAVMA KMAKPKKRNE TLVFSHNAVI AMRDGKLCLM WRVGNLRKSH LVEAHVRAQL LKSRITSEGE YIPLDQIDIN VGFDSGIDRI FLVSPITIVH EIDEDSPLYD LSKQDIDNAD FEIVVILEGM VEATAMTTQC RSSYLANEIL WGHRYEPVLF EEKHYYKVDY SRFHKTYEVP NTPLCSARDL AEKKYILSNA NSFCYENEVA LTSKEEDDSE NGVPESTSTD TPPDIDLHNQ ASVPLEPRPL RRESEI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human KCNJ2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: KCNJ2 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 2 (KCNJ2))

Andere Bezeichnung: KCNJ2 (KCNJ2 Produkte)

Synonyme: KCNJ2 Protein, ATFB9 Protein, HHBIRK1 Protein, HHIRK1 Protein, IRK1 Protein, KIR2.1 Protein, LQT7 Protein, SQT3 Protein, Kir2.1 Protein, Kcnf1 Protein, IRK-1 Protein, potassium voltage-gated channel subfamily J member 2 Protein, potassium inwardly-rectifying channel, subfamily J, member 2 Protein, KCNJ2 Protein, Kcnj2 Protein

Hintergrund: Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium, as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium or cesium.

Molekulargewicht: 49.3 kDa Including tag.

UniProt: P63252