UGCGL2 Protein (AA 28-1516) (His tag)

Produktnummer ABIN3096173

Produktdetails

Target: UGCGL2 (UGT2) (UDP-Glucose Glycoprotein Glucosyltransferase 2 (UGT2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 28-1516

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses UGCGL2 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: TVAASKSVTA HLAAKWPETP LLLEASEFMA EESNEKFWQF LETVQELAIY KQTESDYSYY NLILKKAGQF LDNLHINLLK FAFSIRAYSP AIQMFQQIAA DEPPPDGCNA FVVIHKKHTC KINEIKKLLK KAASRTRPYL FKGDHKFPTN KENLPVVILY AEMGTRTFSA FHKVLSEKAQ NEEILYVLRH YIQKPSSRKM YLSGYGVELA IKSTEYKALD DTQVKTVTNT TVEDETETNE VQGFLFGKLK EIYSDLRDNL TAFQKYLIES NKQMMPLKVW ELQDLSFQAA SQIMSAPVYD SIKLMKDISQ NFPIKARSLT RIAVNQHMRE EIKENQKDLQ VRFKIQPGDA RLFINGLRVD MDVYDAFSIL DMLKLEGKMM NGLRNLGING EDMSKFLKLN SHIWEYTYVL DIRHSSIMWI NDLENDDLYI TWPTSCQKLL KPVFPGSVPS IRRNFHNLVL FIDPAQEYTL DFIKLADVFY SHEVPLRIGF VFILNTDDEV DGANDAGVAL WRAFNYIAEE FDISEAFISI VHMYQKVKKD QNILTVDNVK SVLQNTFPHA NIWDILGIHS KYDEERKAGA SFYKMTGLGP LPQALYNGEP FKHEEMNIKE LKMAVLQRMM DASVYLQREV FLGTLNDRTN AIDFLMDRNN VVPRINTLIL RTNQQYLNLI STSVTADVED FSTFFFLDSQ DKSAVIAKNM YYLTQDDESI ISAVTLWIIA DFDKPSGRKL LFNALKHMKT SVHSRLGIIY NPTSKINEEN TAISRGILAA FLTQKNMFLR SFLGQLAKEE IATAIYSGDK IKTFLIEGMD KNAFEKKYNT VGVNIFRTHQ LFCQDVLKLR PGEMGIVSNG RFLGPLDEDF YAEDFYLLEK ITFSNLGEKI KGIVENMGIN ANNMSDFIMK VDALMSSVPK RASRYDVTFL RENHSVIKTN PQENDMFFNV IAIVDPLTRE AQKMAQLLVV LGKIINMKIK LFMNCRGRLS EAPLESFYRF VLEPELMSGA NDVSSLGPVA KFLDIPESPL LILNMITPEG WLVETVHSNC DLDNIHLKDT EKTVTAEYEL EYLLLEGQCF DKVTEQPPRG LQFTLGTKNK PAVVDTIVMA HHGYFQLKAN PGAWILRLHQ GKSEDIYQIV GHEGTDSQAD LEDIIVVLNS FKSKILKVKV KKETDKIKED ILTDEDEKTK GLWDSIKSFT VSLHKENKKE KDVLNIFSVA SGHLYERFLR IMMLSVLRNT KTPVKFWLLK NYLSPTFKEV IPHMAKEYGF RYELVQYRWP RWLRQQTERQ RIIWGYKILF LDVLFPLAVD KIIFVDADQI VRHDLKELRD FDLDGAPYGY TPFCDSRREM DGYRFWKTGY WASHLLRRKY HISALYVVDL KKFRRIGAGD RLRSQYQALS QDPNSLSNLD QDLPNNMIYQ VAIKSLPQDW LWCETWCDDE SKQRAKTIDL CNNPKTKESK LKAAARIVPE WVEYDAEIRQ LLDHLENKKQ DTILTHDEL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human UGGT2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: UGCGL2 (UGT2) (UDP-Glucose Glycoprotein Glucosyltransferase 2 (UGT2))

Andere Bezeichnung: UGGT2 (UGT2 Produkte)

Synonyme: UGCGL2 Protein, ugcgl2 Protein, im:7146988 Protein, wu:fi13a08 Protein, HUGT2 Protein, UGT2 Protein, 1810064L21Rik Protein, 3110001A05Rik Protein, 3110027P15Rik Protein, A230065J02Rik Protein, AW047562 Protein, Ugcgl2 Protein, UDP-glucose glycoprotein glucosyltransferase 2 Protein, UGGT2 Protein, uggt2 Protein, Uggt2 Protein

Hintergrund: Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation (By similarity). {ECO:0000250}.

Molekulargewicht: 173.0 kDa Including tag.

UniProt: Q9NYU1

Pathways: SARS-CoV-2 Protein Interaktom