TTLL8 Protein (AA 1-850) (Strep Tag)

Produktnummer ABIN3096145

Produktdetails

Target: TTLL8 (Tubulin Tyrosine Ligase-Like Family, Member 8 (TTLL8))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-850

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses TTLL8 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MEPERKGLSL ASSSDGDGRE ENKLKQGISQ DLASSSRLDR YKIARQLTEK AIKEKKIFSI YGHYPVVRAA LRRKGWVEKK FHFLPKVIPD VEDEGARVND DTCAKVKENQ EMALEKTDNI HDVMSRLVKN EMPYLLWTIK RDIIDYHSLT YDQMLNHYAK TASFTTKIGL CVNMRSLPWY VPANPDSFFP RCYSLCTESE QQEFLEDFRR TMASSILKWV VSHQSCSRSS RSKPRDQREE AGSSDLSSRQ DAENAEAKLR GLPGQLVDIA CKVCQAYLGQ LEHEDIDTSA DAVEDLTEAE WEDLTQQYYS LVHGDAFISN SRNYFSQCQA LLNRITSVNP QTDIDGLRNI WIIKPAAKSR GRDIVCMDRV EEILELAAAD HPLSRDNKWV VQKYIETPLL ICDTKFDIRQ WFLVTDWNPL TIWFYKESYL RFSTQRFSLD KLDSAIHLCN NAVQKYLKND VGRSPLLPAH NMWTSTRFQE YLQRQGRGAV WGSVIYPSMK KAIAHAMKVA QDHVEPRKNS FELYGADFVL GRDFRPWLIE INSSPTMHPS TPVTAQLCAQ VQEDTIKVAV DRSCDIGNFE LLWRQPVVEP PPFSGSDLCV AGVSVRRARR QVLPVCNLKA SASLLDAQPL KARGPSAMPD PAQGPPSPAL QRDLGLKEEK GLPLALLAPL RGAAESGGAA QPTRTKAAGK VELPACPCRH VDSQAPNTGV PVAQPAKSWD PNQLNAHPLE PVLRGLKTAE GALRPPPGGK GEGTVCSRLP HHGHHVAACQ TTGTTWDGGP GVCFLRQLLA SELPMGPGLP RDPRAPPCLV CRGLLPPAGP CKRCRSFCAA VLQGASFVRL GGRSCSPRTP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TTLL8 (Tubulin Tyrosine Ligase-Like Family, Member 8 (TTLL8))

Andere Bezeichnung: TTLL8 (TTLL8 Produkte)

Synonyme: 1700019P01Rik Protein, 4933401B17 Protein, tubulin tyrosine ligase like 8 Protein, tubulin tyrosine ligase-like family, member 8 Protein, TTLL8 Protein, Ttll8 Protein

Hintergrund: Protein monoglycylase TTLL8 (EC 6.3.2.-) (Tubulin--tyrosine ligase-like protein 8),FUNCTION: Monoglycylase which modifies both tubulin and non-tubulin proteins, adding a single glycine to the gamma-carboxyl groups of specific glutamate residues to generate monoglycine side chains within the C-terminal tail of target proteins. Not involved in elongation step of the polyglycylation reaction. Preferentially monoglycylates alpha-tubulin over beta-tubulin. Together with TTLL3, mediates microtubule glycylation of primary and motile cilia, which is essential for their stability and maintenance. Together with TTLL3, glycylates sperm flagella which regulates axonemal dynein motor activity, thereby controlling flagellar beat, directional sperm swimming and male fertility. Monoglycylates non-tubulin proteins such as ANP32A, ANP32B, SET, NCL and NAP1. {ECO:0000250|UniProtKB:A4Q9F1}.

Molekulargewicht: 94.7 kDa

UniProt: A6PVC2