IGHMBP2 Protein (AA 2-993) (His tag)

Produktnummer ABIN3095463

Produktdetails

Target: IGHMBP2 (Immunoglobulin mu Binding Protein 2 (IGHMBP2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-993

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses IGHMBP2 Protein ist gelabelt mit His tag.

Applikation: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: ASAAVESFVT KQLDLLELER DAEVEERRSW QENISLKELQ SRGVCLLKLQ VSSQRTGLYG RLLVTFEPRR YGSAAALPSN SFTSGDIVGL YDAANEGSQL ATGILTRVTQ KSVTVAFDES HDFQLSLDRE NSYRLLKLAN DVTYRRLKKA LIALKKYHSG PASSLIEVLF GRSAPSPASE IHPLTFFNTC LDTSQKEAVL FALSQKELAI IHGPPGTGKT TTVVEIILQA VKQGLKVLCC APSNIAVDNL VERLALCKQR ILRLGHPARL LESIQQHSLD AVLARSDSAQ IVADIRKDID QVFVKNKKTQ DKREKSNFRN EIKLLRKELK EREEAAMLES LTSANVVLAT NTGASADGPL KLLPESYFDV VVIDECAQAL EASCWIPLLK ARKCILAGDH KQLPPTTVSH KAALAGLSLS LMERLAEEYG ARVVRTLTVQ YRMHQAIMRW ASDTMYLGQL TAHSSVARHL LRDLPGVAAT EETGVPLLLV DTAGCGLFEL EEEDEQSKGN PGEVRLVSLH IQALVDAGVP ARDIAVVSPY NLQVDLLRQS LVHRHPELEI KSVDGFQGRE KEAVILSFVR SNRKGEVGFL AEDRRINVAV TRARRHVAVI CDSRTVNNHA FLKTLVEYFT QHGEVRTAFE YLDDIVPENY SHENSQGSSH AATKPQGPAT STRTGSQRQE GGQEAAAPAR QGRKKPAGKS LASEAPSQPS LNGGSPEGVE SQDGVDHFRA MIVEFMASKK MQLEFPPSLN SHDRLRVHQI AEEHGLRHDS SGEGKRRFIT VSKRAPRPRA ALGPPAGTGG PAPLQPVPPT PAQTEQPPRE QRGPDQPDLR TLHLERLQRV RSAQGQPASK EQQASGQQKL PEKKKKKAKG HPATDLPTEE DFEALVSAAV KADNTCGFAK CTAGVTTLGQ FCQLCSRRYC LSHHLPEIHG CGERARAHAR QRISREGVLY AGSGTKNGSL DPAKRAQLQR RLDKKLSELS NQRTSRRKER GT
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human IGHMBP2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: IGHMBP2 (Immunoglobulin mu Binding Protein 2 (IGHMBP2))

Andere Bezeichnung: IGHMBP2 (IGHMBP2 Produkte)

Synonyme: NV12364 Protein, CATF1 Protein, HCSA Protein, HMN6 Protein, SMARD1 Protein, SMUBP2 Protein, ZFAND7 Protein, AEP Protein, Catf1 Protein, RIPE3b1 Protein, Smbp-2 Protein, Smbp2 Protein, Smubp2 Protein, nmd Protein, sma Protein, RIP-1 Protein, RIP1 Protein, immunoglobulin mu binding protein 2 Protein, IGHMBP2 Protein, Ighmbp2 Protein, ighmbp2 Protein

Hintergrund: 5' to 3' helicase that unwinds RNA and DNA duplices in an ATP-dependent reaction. Acts as a transcription regulator. Required for the transcriptional activation of the flounder liver-type antifreeze protein gene. Exhibits strong binding specificity to the enhancer element B of the flounder antifreeze protein gene intron. Binds to the insulin II gene RIPE3B enhancer region. May be involved in translation (By similarity). DNA-binding protein specific to 5'-phosphorylated single-stranded guanine-rich sequence related to the immunoglobulin mu chain switch region. Preferentially binds to the 5'-GGGCT-3' motif. Interacts with tRNA-Tyr. Stimulates the transcription of the human neurotropic virus JCV. {ECO:0000250, ECO:0000269|PubMed:19158098, ECO:0000269|PubMed:19299493}.

Molekulargewicht: 110.0 kDa Including tag.

UniProt: P38935