SBNO2 Protein (AA 1-1366) (Strep Tag)

Produktnummer ABIN3095304

Produktdetails

Target: SBNO2 (Strawberry Notch Homolog 2 (SBNO2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1366

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SBNO2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MLAVGPAMDR DYPQHEPPPA GSLLYSPPPL QSAMLHCPYW NTFSLPPYPA FSSDSRPFMS SASFLGSQPC PDTSYAPVAT ASSLPPKTCD FAQDSSYFED FSNISIFSSS VDSLSDIVDT PDFLPADSLN QVSTIWDDNP APSTHDKLFQ LSRPFAGFED FLPSHSTPLL VSYQEQSVQS QPEEEDEAEE EEAEELGHTE TYADYVPSKS KIGKQHPDRV VETSTLSSVP PPDITYTLAL PSDSGALSAL QLEAITYACQ QHEVLLPSGQ RAGFLIGDGA GVGKGRTVAG VILENHLRGR KKALWFSVSN DLKYDAERDL RDIEATGIAV HALSKIKYGD TTTSEGVLFA TYSALIGESQ AGGQHRTRLR QILDWCGEAF EGVIVFDECH KAKNAGSTKM GKAVLDLQNK LPLARVVYAS ATGASEPRNM IYMSRLGIWG EGTPFRNFEE FLHAIEKRGV GAMEIVAMDM KVSGMYIARQ LSFSGVTFRI EEIPLAPAFE CVYNRAALLW AEALNVFQQA ADWIGLESRK SLWGQFWSAH QRFFKYLCIA AKVRRLVELA REELARDKCV VIGLQSTGEA RTREVLGEND GHLNCFVSAA EGVFLSLIQK HFPSTKRKRD RGAGSKRKRR PRGRGAKAPR LACETAGVIR ISDDSSTESD PGLDSDFNSS PESLVDDDVV IVDAVGLPSD DRGPLCLLQR DPHGPGVLER VERLKQDLLD KVRRLGRELP VNTLDELIDQ LGGPQRVAEM TGRKGRVVSR PDGTVAFESR AEQGLSIDHV NLREKQRFMS GEKLVAIISE ASSSGVSLQA DRRVQNQRRR VHMTLELPWS ADRAIQQFGR THRSNQVSAP EYVFLISELA GERRFASIVA KRLESLGALT HGDRRATESR DLSKYNFENK YGTRALHCVL TTILSQTENK VPVPQGYPGG VPTFFRDMKQ GLLSVGIGGR ESRNGCLDVE KDCSITKFLN RILGLEVHKQ NALFQYFSDT FDHLIEMDKR EGKYDMGILD LAPGIEEIYE ESQQVFLAPG HPQDGQVVFY KISVDRGLKW EDAFAKSLAL TGPYDGFYLS YKVRGNKPSC LLAEQNRGQF FTVYKPNIGR QSQLEALDSL RRKFHRVTAE EAKEPWESGY ALSLTHCSHS AWNRHCRLAQ EGKDCLQGLR LRHHYMLCGA LLRVWGRIAA VMADVSSSSY LQIVRLKTKD RKKQVGIKIP EGCVRRVLQE LRLMDADVKR RQAPALGCPA PPAPRPLALP CGPGEVLDLT YSPPAEAFPP PPHFSFPAPL SLDAGPGVVP LGTPDAQADP AALAHQGCDI NFKEVLEDML RSLHAGPPSE GALGEGAGAG GAAGGGPERQ SVIQFSPPFP GAQAPL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SBNO2 (Strawberry Notch Homolog 2 (SBNO2))

Andere Bezeichnung: SBNO2 (SBNO2 Produkte)

Synonyme: KIAA0963 Protein, SNO Protein, STNO Protein, BC019206 Protein, F730040C21 Protein, Sno Protein, Stno Protein, strawberry notch homolog 2 Protein, strawberry notch homolog 2 (Drosophila) Protein, SBNO2 Protein, Sbno2 Protein

Hintergrund: Protein strawberry notch homolog 2,FUNCTION: Acts as a transcriptional coregulator, that can have both coactivator and corepressor functions. Inhibits the DCSTAMP-repressive activity of TAL1, hence enhancing the access of the transcription factor MITF to the DC-STAMP promoter in osteoclast. Plays a role in bone homeostasis, required as a positive regulator in TNFSF11//RANKL-mediated osteoclast fusion via a DCSTAMP-dependent pathway. May also be required in the regulation of osteoblast differentiation (By similarity). Involved in the transcriptional corepression of NF-kappaB in macrophages (PubMed:18025162). Plays a role as a regulator in the pro-inflammatory cascade (PubMed:18025162). {ECO:0000250|UniProtKB:Q7TNB8, ECO:0000269|PubMed:18025162}.

Molekulargewicht: 150.3 kDa

UniProt: Q9Y2G9