RPE65 Protein (AA 2-533) (His tag)

Produktnummer ABIN3095121

Produktdetails

Target: RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-533

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses RPE65 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: SIQVEHPAGG YKKLFETVEE LSSPLTAHVT GRIPLWLTGS LLRCGPGLFE VGSEPFYHLF DGQALLHKFD FKEGHVTYHR RFIRTDAYVR AMTEKRIVIT EFGTCAFPDP CKNIFSRFFS YFRGVEVTDN ALVNVYPVGE DYYACTETNF ITKINPETLE TIKQVDLCNY VSVNGATAHP HIENDGTVYN IGNCFGKNFS IAYNIVKIPP LQADKEDPIS KSEIVVQFPC SDRFKPSYVH SFGLTPNYIV FVETPVKINL FKFLSSWSLW GANYMDCFES NETMGVWLHI ADKKRKKYLN NKYRTSPFNL FHHINTYEDN GFLIVDLCCW KGFEFVYNYL YLANLRENWE EVKKNARKAP QPEVRRYVLP LNIDKADTGK NLVTLPNTTA TAILCSDETI WLEPEVLFSG PRQAFEFPQI NYQKYCGKPY TYAYGLGLNH FVPDRLCKLN VKTKETWVWQ EPDSYPSEPI FVSHPDALEE DDGVVLSVVV SPGAGQKPAY LLILNAKDLS EVARAEVEIN IPVTFHGLFK KS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human RPE65 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))

Andere Bezeichnung: RPE65 (RPE65 Produkte)

Synonyme: LCA2 Protein, RP20 Protein, mRPE65 Protein, rd12 Protein, sRPE65 Protein, 65kDa Protein, A930029L06Rik Protein, Mord1 Protein, DRPE65 Protein, RPE65 Protein, anon-WO0148162.1 Protein, anon-WO0148163.1 Protein, beta-diox Protein, DmelCG9347 Protein, CG9347 Protein, MGC52887 Protein, MGC154296 Protein, MGC85437 Protein, rpe65 Protein, RPE56c Protein, si:ch211-198n5.12 Protein, 13cIMH Protein, RPE56b Protein, rpepb Protein, si:ch211-198n5.5 Protein, wu:fc74c09 Protein, zgc:110538 Protein, RPE56a Protein, rpepa Protein, zgc:73213 Protein, RPE65, retinoid isomerohydrolase Protein, retinal pigment epithelium 65 Protein, neither inactivation nor afterpotential B Protein, retinal pigment epithelium-specific protein 65kDa S homeolog Protein, retinal pigment epithelium-specific protein 65kDa L homeolog Protein, RPE65 homolog Protein, retinal pigment epithelium-specific protein 65kDa Protein, retinal pigment epithelium-specific protein 65c Protein, retinal pigment epithelium-specific protein 65b Protein, retinal pigment epithelium-specific protein 65a Protein, RPE65 Protein, Rpe65 Protein, ninaB Protein, rpe65.S Protein, rpe65.L Protein, rpe65 Protein, rpe65c Protein, rpe65b Protein, rpe65a Protein

Hintergrund: Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association. {ECO:0000269|PubMed:16116091, ECO:0000269|PubMed:21654732}.

Molekulargewicht: 61.8 kDa Including tag.

UniProt: Q16518