Plexin A3 Protein (PLXNA3) (AA 20-1220) (His tag)

Produktnummer ABIN3094645

Produktdetails

Target: Plexin A3 (PLXNA3)

Protein-Typ: Recombinant

Proteineigenschaft: AA 20-1220

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses Plexin A3 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: NRPFRAFVVT DTTLTHLAVH RVTGEVFVGA VNRVFKLAPN LTELRAHVTG PVEDNARCYP PPSMRVCAHR LAPVDNINKL LLIDYAARRL VACGSIWQGI CQFLRLDDLF KLGEPHHRKE HYLSGAQEPD SMAGVIVEQG QGPSKLFVGT AVDGKSEYFP TLSSRKLISD EDSADMFSLV YQDEFVSSQI KIPSDTLSLY PAFDIYYIYG FVSASFVYFL TLQLDTQQTL LDTAGEKFFT SKIVRMCAGD SEFYSYVEFP IGCSWRGVEY RLVQSAHLAK PGLLLAQALG VPADEDVLFT IFSQGQKNRA SPPRQTILCL FTLSNINAHI RRRIQSCYRG EGTLALPWLL NKELPCINTP MQINGNFCGL VLNQPLGGLH VIEGLPLLAD STDGMASVAA YTYRQHSVVF IGTRSGSLKK VRVDGFQDAH LYETVPVVDG SPILRDLLFS PDHRHIYLLS EKQVSQLPVE TCEQYQSCAA CLGSGDPHCG WCVLRHRCCR EGACLGASAP HGFAEELSKC VQVRVRPNNV SVTSPGVQLT VTLHNVPDLS AGVSCAFEAA AENEAVLLPS GELLCPSPSL QELRALTRGH GATRTVRLQL LSKETGVRFA GADFVFYNCS VLQSCMSCVG SPYPCHWCKY RHTCTSRPHE CSFQEGRVHS PEGCPEILPS GDLLIPVGVM QPLTLRAKNL PQPQSGQKNY ECVVRVQGRQ QRVPAVRFNS SSVQCQNASY SYEGDEHGDT ELDFSVVWDG DFPIDKPPSF RALLYKCWAQ RPSCGLCLKA DPRFNCGWCI SEHRCQLRTH CPAPKTNWMH LSQKGTRCSH PRITQIHPLV GPKEGGTRVT IVGDNLGLLS REVGLRVAGV RCNSIPAEYI SAERIVCEME ESLVPSPPPG PVELCVGDCS ADFRTQSEQV YSFVTPTFDQ VSPSRGPASG GTRLTISGSS LDAGSRVTVT VRDSECQFVR RDAKAIVCIS PLSTLGPSQA PITLAIDRAN ISSPGLIYTY TQDPTVTRLE PTWSIINGST AITVSGTHLL TVQEPRVRAK YRGIETTNTC QVINDTAMLC KAPGIFLGRP QPRAQGEHPD EFGFLLDHVQ TARSLNRSSF TYYPDPSFEP LGPSGVLDVK PGSHVVLKGK NLIPAAAGSS RLNYTVLIGG QPCSLTVSDT QLLCDSPSQT GRQPVMVLVG GLEFWLGTLH ISAERALTLP A
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PLXNA3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Plexin A3 (PLXNA3)

Andere Bezeichnung: PLXNA3 (PLXNA3 Produkte)

Synonyme: MGC89495 Protein, PLXNA3 Protein, plxn3 Protein, plxn4 Protein, xap-6 Protein, plexin-A3 Protein, 6.3 Protein, HSSEXGENE Protein, PLXN3 Protein, PLXN4 Protein, XAP-6 Protein, PlexA3 Protein, Plxa3 Protein, Plxn3 Protein, Plxn4 Protein, SEX Protein, mKIAA4078 Protein, plexina3 Protein, plexin A3 Protein, plexin A3 L homeolog Protein, plxna3 Protein, PLXNA3 Protein, plxna3.L Protein, Plxna3 Protein

Hintergrund: Coreceptor for SEMA3A and SEMA3F. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance in the developing nervous system. Regulates the migration of sympathetic neurons, but not of neural crest precursors. Required for normal dendrite spine morphology in pyramidal neurons. May play a role in regulating semaphorin-mediated programmed cell death in the developing nervous system. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.

Molekulargewicht: 132.4 kDa Including tag.

UniProt: P51805

Pathways: Regulation of Cell Size